中华绒螯蟹雌性亲蟹血淋巴生化指标与盐度的关系

设定淡水对照组和两个盐度组(盐度12,盐度25),分别在第0、3、6、12、24、48、72、96、144小时取样,研究盐度对中华绒螯蟹雌性亲蟹6项血淋巴生化指标的影响。结果表明,在0~144 h内,盐度组中华绒螯蟹雌性亲蟹血淋巴总蛋白(TP)含量在前6 h显著高于对照组(P<0.05),96 h后高低盐度组TP水平趋于一致,并保持在略高于对照组的稳定水平。葡萄糖(GLU)含量随盐度的升高而增大,盐度组GLU均呈先升高后降低趋势,盐度25组GLU在72 h达到最大值且显著高于盐度12组和对照组(P<0.05)。盐度12组甘油三酯(TG)含量呈降低趋势且在6~144 h显著低于对照组(P<0.05),72 h后显著低于盐度25组(P<0.05),盐度25组TG呈先下降后上升趋势,且在72 h后恢复至对照水平。盐度组碱性磷酸酶(ALP)活性呈先升高后降低趋势,盐度组乳酸脱氢酶(LDH)活性均在3 h达到最大值,12 h取得最小值。分析认为,中华绒螯蟹雌性亲蟹由淡水进入咸水,其血淋巴TP、GLU、TG含量均发生显著性变化,推测其机体加强能量代谢,加速动用脂类和糖类作为能源物质以应对盐度突变刺激;并最先调节蛋白质代谢过程以响应外界坏境的渗透压变化进行渗透压调节,其机体最终能够适应外界较高盐度的水体。

The relationship between hemolymph biochemical parameters and salinity in female parent Chinese mitten crab (Eriocheir sinensis)

Two salinity concentrations(salinity 12,salinity 25)and a control group of fresh water were set up in the experiment.The samples were respectively taken at 0,3,6,12,24,48,72,96,and 144 h to study the effects of salinity changes on the hemolymph biochemical parameters of the female parent Chinese mitten crab (Eriocheir sinensis).The results showed that the values of total protein(TP) in salinity concentrations were significantly higher than that of the control group during the first 6 hours(P<0.05)while the values of TP in two salinity concentrations reached the same level and kept a little higher than that of the control group after 96 hours.The values of glucose(GLU)increased with the increasing of salinity concentration,and the values of GLU both increased first and then declined in the salinity concentrations.The values of GLU in salinity 25 reached the maximum level at 72 h and were significantly higher than that of the other two groups (P<0.05).The values of triglycerides(TG)in salinity 12 declined gradually,and were significantly lower than that of control group during 6-144 h(P<0.05),yet were significantly lower than that of salinity 25 after 72 h(P<0.05).The values of TG in salinity 25 declined first and then increased,and reached the same level as control group after 72 h.The values of alkaline phosphatase(ALP)both increased first and then declined in the two salinity concentrations.The values of lactate dehydrogenase(LDH) in the two salinity concentrations both reached the maximum values at 3 h and reached the minimum at 12 h.The result showed hemolymph TP,GLU,and TG all changed significantly during travelling from fresh water to saline water in parent Chinese mitten crab.It could be concluded that(1) the parent Chinese mitten crab enhanced the process of energy metabolism to deal with the sudden change of salinity and increased the use of lipids and carbohydrates as energy source.(2) the parent Chinese mitten crab first regulated protein metabolism in response to the changes of external osmotic pressure and finally adapted to the high-salinity water.