| 菲律宾蛤仔过敏原可视化抗体微阵列玻片的检测方法 |
| |
| 引用本文： | 李小燕,李振兴,林洪,SAMEE Haider,张轶群.菲律宾蛤仔过敏原可视化抗体微阵列玻片的检测方法[J].水产学报,2010,34(3):422-427. |
| |
| 作者姓名： | 李小燕 李振兴 林洪 SAMEE Haider 张轶群 |
| |
| 作者单位： | 1. 中国海洋大学水产品安全性实验室,山东,青岛,266003
2. Food and Marine Research Center, PCSIR Laboratories Complex, Off University Road, Karachi, Pakistan |
| |
| 基金项目： | 国家自然科学基金项目(30800859); 国家“八六三”高技术研究发展计划(2006AA09Z427) |
| |
| 摘 要： | 采用辣根过氧化物酶(HRP)与3,3′,5,5′—四甲基联苯胺(TMB)—H2O2作为信号示踪系统,建立了一种可视化抗体微阵列检测菲律宾蛤仔过敏原的方法。对孵育时间、抗体浓度等免疫条件进行了优化。采用改良双抗体夹心法,将菲律宾蛤仔过敏原的兔多克隆抗体固定于琼脂糖三维芯片上,依次加入待检样品、菲律宾蛤仔鼠多克隆抗体和HRP-羊抗鼠IgG,孵育后加TMB显色,肉眼观察后,用平板扫描仪获取扫描图象,采用GenePixPro6.0软件分析灰度值。试验结果表明,该方法最低可检出10ng/mL的菲律宾蛤仔过敏原,片内平均变异系数(CV)为5.99%,片间为10.3%;香肠及蟹棒中3个不同浓度的加标回收率为73.54%～95.44%;4℃存放4个月内抗体微阵列玻片活性保持稳定。该方法不需要大型精密仪器,结果直观可见,可以发展为对多种过敏原进行同时检测,具有良好的实用和推广价值。
|
| 关 键 词： | 菲律宾蛤仔 过敏原 夹心法 抗体微阵列 可视化 |
| 收稿时间： | 2009/7/14 0:00:00 |
| 修稿时间： | 2009/12/9 0:00:00 |
Development of visible antibody microarray chips for detecting mottled calm (Ruditapes philippinarum) allergen |
| |
| LI Xiaoyan,LI Zhenxing,LIN Hong,SAMEE Haider and ZHANG Yiqun.Development of visible antibody microarray chips for detecting mottled calm (Ruditapes philippinarum) allergen[J].Journal of Fisheries of China,2010,34(3):422-427. |
| |
| Authors: | LI Xiaoyan LI Zhenxing LIN Hong SAMEE Haider and ZHANG Yiqun |
| |
| Institution: | LI Xiao-yan1,LI Zhen-xing1,LIN Hong 1,SAMEE Haider2,ZHANG Yi-qun1 (1.Aquatic Products Safety Laboratory,Ocean University of China,Qingdao 266003,China) 2.Food , Marine Research Center,PCSIR Laboratories Complex,Off University Road,Karachi,Pakistan) |
| |
| Abstract: | Ruditapes philippinarum has a widespread consumption in China. It is enjoyed for the uniqueness of its flavour and has a wide variety of applications in foods,such as soup and flavouring. Associated with the diversiform consumption of Ruditapes philippinarum is the general increase of allergic disorder. To provide guarantee for the allergy sufferers,it is important to establish a more convenient method for the detection of food allergen. A visible antibody microarray for detecting Ruditapes philippinarum al... |
| |
| Keywords: | Ruditapes philippinarum allergen sandwich antibody microarray visualization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
