黄芪多糖和当归多糖对维氏气单胞菌诱导鲫细胞凋亡的影响

为研究黄芪多糖(APS)和当归多糖(ASP)对维氏气单胞菌诱导鲫细胞凋亡的影响,实验设阴性对照组、阳性对照组,黄芪多糖组和当归多糖组,通过对鲫用维氏气单胞菌攻毒处理,用流式细胞仪测定血细胞的凋亡比例和细胞周期变化,并用荧光显微镜和透射电镜观察凋亡细胞的形态。结果显示,维氏气单胞菌攻毒后阳性对照组鲫血细胞的细胞凋亡率均极显著高于多糖组和阴性对照组;多糖组能显著降低鲫的细胞凋亡率且黄芪多糖组效果更显著;攻毒后鲫肝细胞和肾脏淋巴细胞出现染色质凝集、细胞核固缩边集和细胞空泡化及凋亡小体;同阴性对照组相比;维氏气单胞菌攻毒可以引起鲫血细胞周期中S/G2+M期细胞比例极显著下降,sub-G1极显著升高,抑制细胞分裂诱发凋亡;多糖组则G0/G1期细胞极显著降低,S/G2+M期细胞极显著升高,sub-G1极显著降低,促进细胞分裂抑制凋亡。黄芪多糖和当归多糖添加量在1%时能抑制维氏气单胞菌攻毒引起的细胞凋亡。

Effect of Astragalus polysaccharide and Angelica sinensis polysaccharide on apoptosis of Carassius auratus induced by Aeromonas veronii

The present study aimed to evaluate the effect of Astragalus polysacharide (APS) and Angelica sinensis polysaccharide (ASP) on the cell apoptosis of Carassius auratus induced by Aeromonas veronii. Negative control group, positive control group, APS group and APS group (four groups) were set up in this experiment. Experimental fish was infected with A. veronii. The changes of apoptosis rate and cell cycle in blood cells were determined by flow cytometer. The morphology of apoptosis cells were observed by fluorescence microscopy and transmission electron microscopy (TEM). The results showed that the blood cell apoptosis rate of the positive control group was significantly higher than those of two polysaccharide groups and negative control group after being infected with A. veronii. Polysaccharide groups could significantly reduce the apoptosis rate and APS group had more significant effect than ASP group. Karyopyknosis appeared in the liver cells and the head kidney lymphocytes of crucian carp. Besides,chromatic agglutination adjacent to the nuclear membrane and apoptotic bodies appeared after the infection. Compared with the negative control group, the A. veronii infection could significantly decrease the percentage of S/G2+M phase cells and increase sub-G1 phase cells in crucian carp blood cell cycle and the infection had an effect on inhibiting cell division and inducing apoptosis. Compared with the negative control group, the proportion of G0/G1 and sub-G1 cells in the polysaccharide group was significantly reduced, and the proportion of S/G2+M phase cells was significantly increased, which indicated that polysaccharide groups can promote cell division and inhibition of apoptosis. Based on the above results, we found that APS and ASP could effectively inhibit crucian carp apoptosis induced by A. veronii when the addition was 1%.