| 中国对虾血淋巴抗凝剂的筛选 |
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| 引用本文： | 蒋琼.中国对虾血淋巴抗凝剂的筛选[J].水产学报,2001,25(4):359-362,T001. |
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| 作者姓名： | 蒋琼 |
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| 作者单位： | 中国科学院海洋研究所, |
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| 基金项目： | 国家"973'课题资助项目(G1999012005);国家"95"攻关计划课题中子专题(96005301) |
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| 摘 要： | 依据中国对虾血细胞电镜照片中细胞整体形态和细胞器、细胞核的变化,研究对虾抗凝剂成分配比,初步确认了一种理想的抗凝剂配方NaCl450mM,KCl100mM,EDTA,Na210mM,HEPES10mM(pH7.45)。它能够良好的保持细胞的整体形态以及细胞器、细胞核的形态。
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| 关 键 词： | 中国对虾 血细胞 抗凝剂 筛选 |
| 文章编号： | 1000-0615(2001)04-0359-05 |
| 收稿时间： | 2014/4/15 0:00:00 |
| 修稿时间： | 2014/4/15 0:00:00 |
Selection of Anticoagulant to the Hemolymph of Penaeus chinesis |
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| JIANG Qiong.Selection of Anticoagulant to the Hemolymph of Penaeus chinesis[J].Journal of Fisheries of China,2001,25(4):359-362,T001. |
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| Authors: | JIANG Qiong |
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| Institution: | Institute of Oceanology, CAS, Qingdao 266071, China |
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| Abstract: | The morphology of the whole hemocyte and the variation of organelle and nucleus were observed by TEM in order to determine the composition of shrimp anticoagulant. A confirmed desirable anticoagulant is composed of NaCl 450mM,KCl 10mM,EDTA.Na 2 10mM,HEPES 10mM ( pH 7.45),which can maintain successfully the form of the whole hemocyte,organelle and nucleus. |
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| Keywords: | Penaeus chinensis hemocyte anticoagulant selection |
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