基于线粒体控制区的许氏平鲉养殖群体与野生群体比较研究

为研究许氏平鲉养殖群体与野生群体遗传结构及遗传多样性状况,采用PCR扩增获得许氏平鲉线粒体DNA控制区高变区片段,并对其进行比对分析。结果显示,在长度为451 bp的线粒体控制区片段中,养殖群体单倍型多样度(0.540±0.067~0.815±0.021)明显低于野生群体(0.883±0.053~0.944±0.028),而核苷酸多样度(0.001±0.001~0.007±0.004)与野生群体(0.004±0.003~0.007±0.004)相差不大,遗传多样性水平均较低。在52个单倍型中,养殖群体仅占12个,且有6个单倍型与野生群体共享。群体间遗传分化指数和AMOVA分析结果显示,养殖群体和野生群体之间以及养殖群体之间的遗传分化较大,而野生群体间遗传变异较小,组群间的遗传分化较小且不显著(ΦCT=-0.013;P0.05)。单倍型最小跨度树和NJ系统发育树均未检测到明显的谱系结构。

Comparative analysis of genetic variation in cultured and wild populations of black rockfish(Sebastes schlegelii) based on mitochondrial DNA control region

To investigate the genetic variation and diversity of black rockfish Sebastes schlegelii among four cultured populations and six wild populations,the 451bp hypervariable fragments of mitochondrial DNA control region were sequenced and analyzed.The results revealed that the gene diversity indices of cultured populations ranged from 0.540±0.067 to 0.815±0.021,which were significantly lower than those of wild populations(0.883±0.053-0.944±0.028),while the values of nucleotide diversity for both cultured and wild populations were at low level.Among the 52 haplotypes defined,only 12 of them were in the possession of cultured populations which shared 6 haplotypes with the wild populations.The results of pairwise F_st and AMOVA indicated that significant genetic differentiation existed among cultured populations,as well as between wild and cultured populations,which was contrary to that detected among wild populations.No significant genetic differentiations were detected between cultured and wild groups.Significant genealogical structure was not detected either corresponding to the cultured or wild populations based on the MST and NJ analyses.