肌醇对氨氮应激下团头鲂幼鱼免疫的影响

为了研究氨氮应激下肌醇对团头鲂幼鱼免疫的影响,实验选择初均重为(3.40±0.07)g的健康团头鲂幼鱼450尾,随机分为6组,每组3个重复,分别投喂含肌醇0、101.2、202.3、404.8、809.1和1 616.4 mg/kg的精制饲料,实验期为90 d。结果表明:与对照组相比,氨氮应激前,404.8 mg/kg肌醇添加组显著提高了团头鲂幼鱼淋巴细胞百分比、补体3、补体4和血液呼吸爆发活性(P0.05);氨氮应激12 h,404.8和809.1 mg/kg肌醇添加组的血液白细胞、红细胞、淋巴细胞百分比、血红蛋白和血清补体3、补体4水平显著升高(P0.05),皮质醇水平显著降低(P0.05),202.3和404.8 mg/kg肌醇添加组的血细胞呼吸爆发活性显著提高(P0.05);氨氮应激72 h,404.8 mg/kg肌醇添加组的血液白细胞、红细胞、淋巴细胞百分比、血红蛋白、血清补体3和血细胞呼吸爆发活性显著升高(P0.05),皮质醇水平显著下降(P0.05),809.1 mg/kg肌醇添加组的血清补体4水平显著升高(P0.05)。研究表明,饲料中添加适量的肌醇(404.8 mg/kg)即可增强团头鲂幼鱼的免疫力,对团头鲂幼鱼抗氨氮应激起到了一定的保护作用。

Effects of dietary inositol on immune function of juvenile Wuchang bream under ammonia stress

This study was designed to determine the effects of dietary inositol on immune function of juvenile Wuchang bream(Megalobrama amblycephala)under ammonia stress.In trial,four hundred and fifty juvenile Wuchang bream with initial body weight of(3.40±0.07)g were randomly allocated to 6 groups(with 3 replicates)fed with purified diet containing inositol 0,101.2,202.3,404.8,809.1 and 1 616.4 mg/kg for 90 days.The results showed that,before ammonia stress,compared to the control group,Lym,C3,C4 and respiratory burst activity of fish fed diet 404.8 mg/kg inositol were significantly higher(P<0.05).Under ammonia stress for 12 hours,the WBC,RBC,Lym,HGB,C3 and C4 were significantly higher in the groups supplemented with 404.8 and 809.1 mg/kg inositol(P<0.05);respiratory burst activity was significantly higher than that of control(P<0.05);cortisol level was significantly decreased(P<0.05).Under ammonia stress for 72 hours,the WBC,RBC,Lym,HGB,C3 and respiratory burst activity fed diet 404.8 mg/kg inositol were significantly higher(P<0.05);cortisol level was significantly decreased(P<0.05);C4 levels was was significantly enhanced in the group supplemented with 809.1 mg/kg inositol(P<0.05).So,it is suggested that ingestion of a basal diet supplemented with inositol(404.8 mg/kg)can enhance immune function and resistance against ammonia stress of juvenile Wuchang bream.