Triploid Induction in the Yellowtail Tetra,Astyanax altiparanae,Using Temperature Shock: Tools for Conservation and Aquaculture |
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| Authors: | Nadya Soares de Macedo Adamov Nivaldo Ferreira do Nascimento Elayna Cristina Silva Maciel Matheus Pereira‐Santos José Augusto Senhorini Leonardo Luiz Calado Mariana Machado Evangelista Laura Satiko Okada Nakaghi Alan Hertz Marín Guerrero Takafumi Fujimoto George Shigueki Yasui |
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| Institution: | 1. Department of Veterinary Medicine – FZEA, University of S?o Paulo, Avenida Duque de Caxias Norte 225, Pirassununga, S?o Paulo, Brazil;2. Laboratory of Fish Biotechnology, National Center for Research and Conservation of Continental Fish, Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga, S?o Paulo, Brazil;3. Aquaculture Center, S?o Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, Jaboticabal, S?o Paulo, Brazil;4. School of Technology, University of Campinas – UNICAMP, Limeira, S?o Paulo, Brazil;5. Faculty of Fisheries Sciences, Hokkaido University, 3‐1‐1 Minato‐cho, Hakodate, Japan |
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| Abstract: | Triploidization is an interesting tool to produce sterile fish. In the yellowtail tetra, Astyanax altiparanae, this can be applied for aquaculture and surrogate technologies. In this study, we compared the efficacy of cold (2 C) or heat shock (38 C, 40 C, and 42 C) on triploid induction in the yellowtail tetra. The eggs were treated with cold or heat shock, 2 min postfertilization (30 min in cold shock or 2 min in heat shock). Intact embryos served as the control group. Ploidy status was confirmed by karyotyping, flow cytometry, and nuclear diameter of erythrocytes. The hatching rate decreased after cold shock (12.69 ± 15.76%) and heat shock at 42 C (0.35 ± 0.69%) in comparison with the control group (63.19 ± 16.82%). At 38 C and 40 C, hatching rates (61.29 ± 17.73% and 61.75 ± 22.1%, respectively) were not decreased. Only one triploid arose at 38 C (1/80). At 40 C, a high number of triploids arose (72/78). At 42 C, very few embryos developed into the hatching stage. A large number of haploid individuals arose after cold shock (61/75), with only one triploid. Our results indicate that heat shocking of embryos at 40 C is optimum for triploid production in the yellowtail tetra. |
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