Interactive Effects of Vitamin C and E Supplementation on Growth,Fatty Acid Composition,and Lipid Peroxidation of Sea Cucumber,Apostichopus japonicus,Fed with Dietary Oxidized Fish Oil |
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| Authors: | Jian Gao Shunsuke Koshio Manabu Ishikawa Saichiro Yokoyama Daisuke Nose Tongjun Ren |
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| Institution: | 1. Laboratory of Aquatic Animal Nutrition, College of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Huazhong Agricultural University, Wuhan, China;2. Laboratory of Aquatic Animal Nutrition, Faculty of Fisheries, Kagoshima University, Kagoshima 890–0056, Japan;3. Key Laboratory of Mariculture and Biotechnology, Ministry of Agriculture, Dalian Ocean University, Dalian, China |
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| Abstract: | A feeding trial was conducted to determine the interactive effects of vitamin C (ascorbic acid, AsA) and E (α‐tocopherol, α‐Toc) supplementation with dietary oxidized fish oil (OFO) on the growth performance, whole‐body AsA, and α‐Toc concentrations and fatty acid composition of juvenile sea cucumber. In a 9‐wk feeding trial, juveniles (average weight: 0.6 ± 0.1 g) were cultured in twenty‐four 50‐L tanks (30 juveniles per tank) in triplicate, and fed with eight test diets containing two levels of OFO (8.9 and 156.9 meq/kg) with varying levels of vitamin C (500 and 1000 mg AsA equivalents/kg diet) and E (100 and 200 mg α‐Toc equivalents/kg diet) supplementation, respectively. Body weight gain of sea cucumber was significantly reduced by dietary OFO, while mortality and whole‐body thiobarbituric acid‐reactive substances value were increased significantly. Increasing dietary vitamin C and E levels significantly increased whole‐body α‐Toc and AsA concentrations, respectively. A high level of vitamin E combined with OFO led to consumption of AsA. Even with supplementation of a large dose of vitamin C and/or E in diets, growth performance could not be improved, probably due to the high levels of vitamins in the control diets. |
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