Levels of Vitellogenin in Male Japanese Medaka (Oryzias latipes) Exposed to the Chemotherapeutics: Oxytetracycline,Romet-30®, and Copper Sulfate |
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| Authors: | Kimberly E Tulou Stephen A Smith George J Flick |
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| Institution: | 1. Civil and Environmental Engineering, Virginia Tech , Blacksburg, VA, 24061;2. Biomedical Science &3. Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech , Blacksburg, VA, 24061;4. Food Science and Technology, Virginia Tech , Blacksburg, VA, 24061 |
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| Abstract: | ABSTRACT Japanese medaka (Oryzias latipes) were exposed to oxytetracycline and Romet-30® at daily doses via feed of 0.06 mg, 0.11 mg, and 0.28 mg per gram body weight for 11 days, and at daily doses of 0.05 mg, 0.10 mg, and 0.15 mg per gram body weight for 5 days, respectively. In addition, fish were exposed to copper sulfate in their aquaria water at 1.85 mg/L for 1 h each day (over a span of 1 day, 7 consecutive days, or 1 day in every 3 to 5 days for a total of seven doses). Vitellogenin levels were measured in fish using a sandwich enzyme-linked immunosorbent assay (ELISA). Vitellogenin levels were not induced from exposure to either oxytetracycline or Romet-30® (P > 0.05); however, copper sulfate for 1 h on 1 day showed a decrease in vitellogenin from the control fish and other treatment groups (P = 0.007). Under these test conditions, oxytetracycline, Romet-30, and copper sulfate were not found to act in medaka as estrogenic compounds by inducing vitellogenin. |
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| Keywords: | Vitellogenin medaka oxytetracycline Romet-30 copper sulfate |
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