Tubercular Lesions in Two Spanish Cyprinodontid fishes

A histopathological study of tubercular lesions in Lebias (=Aphanius) ibera and Valencia hispanica (Cyprinodontidae) was carried out using conventional acid-fast staining and immunohistochemical methods. The clinical signs in diseased fish comprised exophthalmos, emaciation and melanosis. Examination of stained sections revealed a systemic granulomatous process associated with positive Zielh–Neelsen bacilli in the lesions. The immunohistochemical staining was positive in all cases and the mycobacterial antigen was detected in affected organs. This revised version was published online in August 2006 with corrections to the Cover Date.