The nucleotide sequence of the gene encoding GCAT from Aeromonas salmonicida ssp. salmonicida

The nucleotide sequence of the gene encoding GCAT (glycerophospholipid: cholesterol acetyltransferase) in Aeromonas salmonicida ssp. salmonicida has been determined. The sequence revealed an open reading frame of 1005 bp encoding 335 amino acids, an 18-amino-acid signal peptide and a 317-amino-acid mature polypeptide with a deduced molecular mass of 35 380 Da and an estimated pI of 6.25. Within the encoding region, the homology with the corresponding gene fromAeromonas hydrophila was 92.9% for the nucleotide sequence and 93.7% for the deduced amino acid sequence.