Validation of a KHV antibody enzyme‐linked immunosorbent assay (ELISA) |
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| Authors: | S M Bergmann Q Wang W Zeng Y Li Y Wang M Matras M Reichert D Fichtner M Lenk T Morin N J Olesen H F Skall P‐Y Lee S Zheng S Monaghan S Reiche W Fuchs M Kotler K Way G Bräuer K Böttcher A Kappe J Kielpinska |
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| Institution: | 1. Friedrich‐Loeffler‐Institut, Federal Research Institute for Animal Health, Greifswald‐Insel Riems, Germany;2. Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences Fisheries Research Institute, Guangzhou, China;3. Department of Fish Diseases, National Veterinary Research Institute, Pulawy, Poland;4. Agence Nationale de Sécurité Sanitaire de l'Alimentation, de l'Environnement et du Travail (ANSES) Ploufragan, Ploufragan, France;5. Section 6. for Diagnostics and Scientific Advice, National Veterinary Institute, Technical University of Denmark, Frederiksberg C, Denmark;7. Department of Research and Development, GeneReach Biotechnology Corporation, Taichung, Taiwan, China;8. Aquatic Vaccine Unit, School of Natural Sciences, Institute of Aquaculture, University of Stirling, Stirling, UK;9. Department of Pathology, The Hebrew University‐Hadassah Medical School, Jerusalem, Israel;10. Centre for Environment, Fisheries and Aquaculture Science, Weymouth, Dorset, UK;11. Fish Health Service Saxony, Dresden, Germany;12. Fish Health Service Thuringia, Bad Langensalza, Germany;13. Department of Faculty of Aquaculture, Food Science and Fisheries, West Pomeranian University of Technology, Szczecin, Poland |
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| Abstract: | Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti‐KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme‐linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs. |
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| Keywords: | carp and koi sera enzyme‐linked immunosorbent assay koi herpesvirus koi herpesvirus antibodies |
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