An in vitro method for testing synergistic action of parasiticides using malachite green and formalin as a model system |
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| Authors: | J P GILBERT J B GRATZEK J BROWN |
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| Institution: | Department of Medical Microbiology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA |
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| Abstract: | Abstract. An in vitro technique was developed to screen drugs for toxicity to laboratory cultured protozoans. Drugs were diluted using standard microtitration equipment. An easily cultured protozoan, Tetrahymena pyriformis , was added to the dilutions of drugs and viability was checked daily for 10 days. Drugs used as a model system to test the technique were formalin and malachite green. Effective concentrations of formalin determined by this in vitro technique were close to recommended therapeutic treatment levels for Ichthyophthirius . Malachite green was effective at concentrations in excess of recommended therapeutic treatment levels for Ichthyophthirius . The synergistic effect of formalin and malachite green was demonstrated. This technique can he of use in determining effectiveness of new drugs as parasiticides. |
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