Susceptibility of cultured juveniles of several marine fish to the sevenband grouper nervous necrosis virus |
| |
Authors: | Tanaka S Kuriyama I Nakai T Miyazaki T |
| |
Institution: | Owase Fisheries Laboratory, Fisheries Research Division, Mie Prefectural Science and Technology Promotion Center, Owase, Mie, Japan;Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, Japan;Faculty of Bioresources, Mie University, Tsu, Mie, Japan |
| |
Abstract: | Piscine nodaviruses (betanodaviruses) have been tentatively divided into four genotypes (SJNNV, RGNNV, TPNNV and BFNNV) and it is suggested that host specificity is different among these genotypes. In the present study, a betanodavirus sevenband grouper nervous necrosis virus (SGNNV)] belonging to the redspotted grouper nervous necrosis virus (RGNNV) genotype, to which most betanodaviruses from warm water fish are identified, was evaluated for its pathogenicity to hatchery-reared juveniles of several marine fish species. When challenged with the virus by a bath method (10(5.1) TCID50 mL(-1)), sevenband grouper, Epinephelus septemfasciatus, Japanese flounder, Paralichthys olivaceus, and tiger puffer, Takifugu rubripes, displayed behavioural abnormalities and mortalities with distinct histopathological signs of viral nervous necrosis and heavily immunostained cells were observed in the central nervous tissues and retina. Bath-challenged rock fish, Sebastiscus marmoratus, and a hybrid of sevenband grouper and kelp grouper, E. moara, did not display any behavioural abnormality or mortality during the experimental period, although many fish showed slight signs of viral infection in nerve cells. Kelp grouper and red sea bream, Pagrus major, showed no behavioural abnormality, mortality or immunohistopathological changes after the virus challenge. These results are, in part, consistent with the natural host range of RGNNV, indicating the complexity in the host specificity of betanodaviruses. |
| |
Keywords: | betanodavirus pathogenicity sevenband grouper susceptibility viral encephalopathy and retinopathy viral nervous necrosis VNN |
本文献已被 PubMed 等数据库收录! |
|