Fertilization by refrigerator stored sperm of the Indian major carp,
Labeo calbasu (Hamilton, 1822) |
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Authors: | Md Mahbubul Hassan Md Nahiduzzaman Shaheed Nasrullah Al Mamun Md Abu Taher Mostafa Ali Reza Hossain |
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Institution: | 1. Department of Fisheries Biology and Genetics, Hajee Mohammad Danesh Science and Technology University, , Dinajpur, Bangladesh;2. Department of Youth Development, Ministry of Youth & Sports, , Bogra, Bangladesh;3. Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, , Mymensingh, Bangladesh |
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Abstract: | This study reports on the spermatological properties, and on the development of a protocol for refrigerator storage (4°C) of Labeo calbasu (Hamilton, 1822) sperm for artificial breeding. Volume, motility, concentration and pH of the freshly collected sperm were 2.21 ± 0.53 (μL g?1 of fish weight) (mean ± SD), 95 ± 1 (%), 1.93 ± 0.44 × 109 (cells mL?1) and 7.56 ± 0.17 respectively. Sperm activation was evaluated at different osmolalities of NaCl solution, and motility ceased completely when osmolality of the extender was ≥287 mOsmol kg?1. Sperm retained motility for 24, 72 and 108 h, after refrigerator storage when sperm were undiluted, suspended in Alsever's solution and suspended in Alsever's solution containing 5% methanol respectively. Fertilization rate of fresh eggs with sperm stored at 4°C in Alsever's solution and Alsever's solution containing 5% methanol was 77% and 60% with a hatching rate of 60% and 43% respectively. The fertilization and hatching success of the stored sperm suggests potential to use refrigeration for transporting genetic material to hatcheries for artificial breeding of L. calbasu in Bangladesh. |
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Keywords: | sperm fertilization hatching refrigerator storage
Labeo calbasu
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