Microsatellite pedigree analysis reveals high variance in reproductive success and reduced genetic diversity in hatchery-spawned northern abalone |
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Authors: | Matthew A Lemay Elizabeth G Boulding |
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Institution: | aDepartment of Integrative Biology, University of Guelph, Ontario, Canada N1G 2W1;bBamfield Marine Sciences Centre, British Columbia, Canada V0R 1B0 |
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Abstract: | The northern (or pinto) abalone, Haliotis kamtschatkana, is a broadcast-spawning marine gastropod that was recently listed as endangered in Canada. To aid in species recovery, a captive-breeding and supplementation program is underway in Barkley Sound, British Columbia. We genotyped first generation progeny for five microsatellite loci and used a pedigree reconstruction program (Pedigree 2.2) to identify their genealogical relationships in the absence of information on parental genotypes. We analyzed progeny from three separate group-spawning events and inferred considerable variation in the number of offspring produced by each parent; in the most severe case a single male sired all the progeny produced during one spawning event. After only one generation of captive-breeding we found a 55–60% reduction in allelic richness and a 17–18% reduction in heterozygosity relative to the diverse wild source population. This study illustrates the difficulty of managing genetic diversity in hatchery populations of a broadcast-spawning species, even when gametes are collected separately from each individual broodstock. |
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Keywords: | Aquaculture Broadcast-spawning Ex situ conservation Haliotis kamtschatkana Population supplementation Sweepstakes hypothesis |
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