| 绵羊血清免疫球蛋白G的纯化及分子量的测定 |
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| 引用本文： | 董淑丽,文凤云,李晓丽,马彦搏.绵羊血清免疫球蛋白G的纯化及分子量的测定[J].畜禽业,2006(14):4-5. |
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| 作者姓名： | 董淑丽 文凤云 李晓丽 马彦搏 |
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| 作者单位： | 河南科技大学动物科技学院,河南,洛阳,471003 |
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| 摘 要： | 采用硫酸胺分级沉淀法、葡聚糖凝胶Sephadex-25层析脱盐、DEAE-纤维素纯化等方法获得绵羊血清免疫球蛋白G。通过SDS-聚丙稀酰胺凝胶电泳法进行分离获得绵羊血清免疫球蛋白G的重链(H链)和轻链(L链),与已知分子量的标准参照蛋白比较,测得重链的分子量约为53000～57000Da,轻链的分子量约为27000～29000Da。
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| 关 键 词： | 绵羊 IgG SDS—PAGE 分子量 |
| 文章编号： | 1008-0414(2006)07(下)-0004-02 |
| 修稿时间： | 2006年5月15日 |
The purification and molecular weight determination of sheep serum Immunoglobulin G |
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| DONG Shu-li,WEN Feng-yun,LI Xiao-li,MA Yan-bo.The purification and molecular weight determination of sheep serum Immunoglobulin G[J].Livestock and Poultry Industry,2006(14):4-5. |
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| Authors: | DONG Shu-li WEN Feng-yun LI Xiao-li MA Yan-bo |
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| Abstract: | Sheep serum Immunoglobulin G(IgG) was purified by ammonium sulfate precipitation, Sephadex-25 chromatography and DEAE-Sepharose column chromatography, respectively. the serum IgG H chain and L chain was then determined by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and its molecular weight was estimated in contrast with the known molecular weight standard protein marks. as a result, the IgG H chain and L chain ranged from 53 000 to 57 000 Da and 27 000 to 29 000 Da, respectively. |
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| Keywords: | IgG SDS-PAGE |
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