鲁西黄牛耳缘组织成纤维细胞的体外培养

实验以鲁西黄牛耳缘组织为研究材料,采用组织块贴壁培养法对其进行了成纤维细胞的体外培养。通过细胞的传代培养、形态学和动力学观察与分析,结果表明,所建立的鲁西黄牛耳缘组织成纤维细胞系具有典型的成纤维细胞形态,胞体均为梭形和不规则三角形,且细胞丰满;细胞核形态为卵圆形,并位于胞质中央,核仁清晰。在细胞生长于繁殖过程中亦经历了潜伏期、指数增生、停滞期和衰退死亡期等4个时期。细胞群体倍增时期为72h。实验结果表明,已成功地培养了鲁西黄牛耳缘组织成纤维细胞。

In Vitro Culture Fibroblast Cell of Luxi Cattle Ear Tissue

A Luxi cattle ear marginal tissue was cultivated by direct adherent culture methods to culture fibroblast in vitro. Observing on subculture, morphology and dynamic growth, the results showed that Luxi cattle ear marginal tissue fibroblast cell is typical fibroblast; cells growing included latent phase, logarithmic growth phase and stagnate phase; population doubling time of cells was 72h.The Luxi cattle ear marginal tissue infinite fibroblast cell was successfully cultured.