慢性氟中毒鸡精子质量测定研究

为探明慢性氟中毒对鸡精子质量的影响,本研究采用饮水染氟方法,人工复制公鸡慢性氟中毒模型,在染氟第三十、五十和七十天分别进行人工采精,测定精子密度、精子活力和精子畸形率。结果表明,慢性氟中毒可导致鸡精子密度和精子活力降低明显,精子畸形率明显升高。对照组精子密度、精子活力和精子畸形率在不同试验时间没有明显差异。而试验组精子密度第五十天和第七十天明显低于第三十天,但第五十天与第七十天无统计学差异;精子活力第七十天也明显低于第三十天,但第三十天与第五十天、第五十天与第七十天之间无统计学差异;试验组精子畸形率不同测定时间都无统计学差异。说明鸡慢性氟中毒呈现一种特定的时间-效应关系,而且精子畸形率可以相对独立的表现鸡对氟的慢性毒性。

Determination the Changes of Rooster Sperm Quality Induced by Chronic Fluorosis

To ascertain the effect of chronic fluorosis is on rooster sperm quality, chronic fluorosis model of roosters was rebuilt artificially by adding F- to the drinking water. In the 30th, 50th and 70th day from the poison reaching time sperms of each animal were collected and the sperm density, sperm vigour and sperm abnormality rate were determined. As a result, chronic fluorosis may cause the reduction of sperm density and sperm vigour and the raise of sperm abnormality rate. The control group has no discrepancy in different testing time. In the experimental group, sperm density of the 50th and 70th day is lower than 30th day, but there is no statistics discrepancy between the 50th and 70th day; and sperm vigour of the 70th day is lower than the 30th day, but there no statistics discrepancy between the 30th and 50th day as well as the 50th and the 70th day; and the sperm abnormality rate has no statistics discrepancy all the testing time. It shows that rooster chronic fluorosis presents a kind of specific time-effect relation, and the sperm abnormality rate takes on a speciality of relatively independence in the process of chronic fluorosis.