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猪圆环病毒2型分离株的基因组克隆、序列测定与分析
引用本文:全滟平,崔尚金,宋木霞,姜艳玲.猪圆环病毒2型分离株的基因组克隆、序列测定与分析[J].畜禽业,2006(3):10-14.
作者姓名:全滟平  崔尚金  宋木霞  姜艳玲
作者单位:中国农科院哈尔滨兽医研究所疫病诊断与流行病学中心,黑龙江,哈尔滨,150001
摘    要:设计合成两对覆盖PCV2基因组全长的引物,对PCV2分离株S2、P11、L进行全基因测序。将所测序列与GenBank上已公布的PCV2毒株序列进行同源性比较,并绘制系统发育进化树。结果显示,所测毒株与其它地域的24株PCV2分离株的基因组序列同源性很高。3个分离株之间的全基因核苷酸同源性在95.4%~99.3%。序列分析表明欧洲分离株和美洲分离株分别构成PCV2的两个大的分支:欧洲系和美洲系。S2、P11属于欧洲系,L属于美洲系。

关 键 词:猪圆环病毒  克隆  序列测定与分析
文章编号:1008-0414(2006)02(上)-0010-05
修稿时间:2005年10月8日

The Molecular Cloned,Sequencing and Analysis of Genome of Porcine circovirus 2
QUAN Yan-ping,CUI Shang-jin,SONG Mu-xia,JIANG Yan-ling.The Molecular Cloned,Sequencing and Analysis of Genome of Porcine circovirus 2[J].Livestock and Poultry Industry,2006(3):10-14.
Authors:QUAN Yan-ping  CUI Shang-jin  SONG Mu-xia  JIANG Yan-ling
Abstract:3 strains of PCV2 were isolated from the affected pigs suffered with PMWS. The full-length genomes of three isolates are amplified with two pairs of primers and cloned. The whole genomes are sequenced and compared. The sequences of three isolates were submitted to GenBank. Genome comparisons show significant differences between PCV1 and PCV2. Phylogenetic analysis of 27 strains reveals overall nucleotide sequence homology of PCV2 is highly, and diversity of different geographic distribution not significant. As showed by the phylogenetic relationships, PCV2 has two large apparent branches: European series and Amercian series. S2?P11 belong to European series,L belongs to Amercian series.
Keywords:Porcine circovirus  Molecular Cloned  Sequencing & Analysis
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