| 一株反硝化细菌的筛选及其反硝化特性的研究 |
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| 引用本文： | 张小玲,梁运祥.一株反硝化细菌的筛选及其反硝化特性的研究[J].淡水渔业,2006,36(5):28-32. |
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| 作者姓名： | 张小玲 梁运祥 |
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| 作者单位： | 华中农业大学生命科学技术学院,农业微生物学国家重点实验室,武汉,430070 |
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| 基金项目： | 浙江省重大科技攻关项目(计划编号:2004C12610) |
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| 摘 要： | 从土壤中分离到一株高活性反硝化菌DNF409,经生理生化和16S rDNA序列分析,初步判断为芽孢杆菌属(Bacillussp.)。在生长的各个阶段,该菌株均具有较强的反硝化活性,最适反硝化碳源为乙醇。在天然养殖水体中,碳氮摩尔比达到8.0∶1、菌体浓度达到108cfu/L时,其反硝化活性即可充分发挥,硝态氮和亚硝态氮的降解率可分别达到94.79%和99.94%。试验表明该菌株在养殖水体的生物脱氮方面具有广阔的应用前景。
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| 关 键 词： | 反硝化作用 芽孢杆菌 亚硝酸盐 C、N摩尔比 |
| 文章编号： | 1000-6907-(2006)05-0028-05 |
| 收稿时间： | 2006-02-20 |
| 修稿时间： | 2006年2月20日 |
Screening of A Strain Denitrobacteria and Its Denitrification Characteristic |
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| ZHANG Xiao-ling,LIANG Yun-xiang.Screening of A Strain Denitrobacteria and Its Denitrification Characteristic[J].Freshwater Fisheries,2006,36(5):28-32. |
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| Authors: | ZHANG Xiao-ling LIANG Yun-xiang |
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| Institution: | College of Life Science and Technology, Huazhong Agricultural University, National Key Laboratory of Agricultural Microbiology, Wuhan 430070 |
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| Abstract: | A newly isolated denitrify bacteria—Bacillus DNF409,possessed high effective denitrification during its whole growth period,and ethanol was the most effective carbon source as the electron donor.When the C,N mole ratio≥8 and the bacterial concentration reached 10~8 cfu/L,the highest degradation efficiencies of nitrate and nitrite were 94.79%and 99.94% respectively after incubation for 60 hours in aquaculture water,which showed the extensive application of Bacillus DNF409 to biodenitrification in the future. |
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| Keywords: | denitrification Bacillus nitrite C N mole ratio |
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