| 中华绒螯蟹卵巢EJ01基因在大肠杆菌BL21（DE3）中的表达 |
| |
| 引用本文： | 陆钟岩,冯金叶,宋大祥,马长艳,郭豫杰,周开亚.中华绒螯蟹卵巢EJ01基因在大肠杆菌BL21（DE3）中的表达[J].淡水渔业,2006,36(5):3-6. |
| |
| 作者姓名： | 陆钟岩 冯金叶 宋大祥 马长艳 郭豫杰 周开亚 |
| |
| 作者单位： | 南京师范大学生命科学学院江苏省生物多样性和生物技术重点实验室,南京210093 |
| |
| 基金项目： | 国家自然科学基金重点项目（No.30130040） |
| |
| 摘 要： | 扩增中华绒螯蟹（Eriocheir sinensis）卵巢EJ01（GenBank检索号：AYl85917）的cDNA全序列得到编码区序列，并将其插入原核表达载体pET28b中，构建成表达质粒pET28b-EJ01。该表达质粒在大肠杆菌（Escherichia coli）BL21（DE3）中经IPTG诱导表达，得到EJ01-HIS6融合蛋白。SDS-PAGE电泳分析表明，该融合蛋白的相对分子质量约为32000。经镍离子柱亲和层析进一步纯化得到纯度较高的EJ01-HIS6融合蛋白。
|
| 关 键 词： | 中华绒螯蟹 EJ01基因 原核表达 EJ01-HIS6融合蛋白 |
| 文章编号： | 1000-6907-(2006)05-0003-04 |
| 收稿时间： | 2006-03-16 |
| 修稿时间： | 2006年3月16日 |
Expression of Eriocheir sinensis Ovarian EJO1 Gene in E.coli BL21 (DE3) |
| |
| LU Zhong-yan, FENG Jin-ye, SONG Da-xiang, MA Chang-yan, GUO Yu-jie, ZHOU Kai-ya.Expression of Eriocheir sinensis Ovarian EJO1 Gene in E.coli BL21 (DE3)[J].Freshwater Fisheries,2006,36(5):3-6. |
| |
| Authors: | LU Zhong-yan FENG Jin-ye SONG Da-xiang MA Chang-yan GUO Yu-jie ZHOU Kai-ya |
| |
| Institution: | Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210097 |
| |
| Abstract: | EJO1(GenBank accessing number:AY185917)is an ovarian gene of Chinese mitten crab(Eriocheir sinensis)presumably involved in the ovary development.The complete mature peptide-coding cDNA fragment of EJO1 was obtained by PCR.The product was inserted into the prokaryotic expression vector,pET28b,to produce the expression vector pET28b-EJO1.The recombinant plasmid was transformed into E.coli BL21(DE3).EJO1-HIS_6 fusion protein was obtained after the addition of IPTG into the growth media.SDS-PAGE analysis revealed that the molecular weight of EJO1-HIS_6 fusion protein was about 32 kDa.Purified fusion protein was obtained after purification with NI-NTA His-Bind resin column. |
| |
| Keywords: | Eriocheir sinensis EJOI gene prokaryotic expression EJO1-HIS6 fusion protein |
| 本文献已被 CNKI 维普 等数据库收录！ |
