牛蛙腹水病病原鉴定和药敏试验

从患病牛蛙(Rana catesbeiana)肝脏、四肢、腹水等组织划线分离,共获得3株菌,通过对菌落形态观察、革兰氏染色镜检及生理生化鉴定,确认其理化性质一致,均为洛菲不动杆菌(Acinetobacter lwoffi)。采取腹部注射和浸泡两种不同方式人工感染试验均证实该菌存在致病性,药敏试验表明,分离菌株W2011-01对左氟沙星、美洛培南、哌拉西林/他唑巴坦、妥布霉素、复方新诺明、庆大霉素等18种药物敏感,对呋喃妥因、环丙沙星、沙拉沙星、乙酰螺旋霉素、青霉素、杆菌肽、痢特灵、利福平、氨苄西林、强力霉素耐药。

Pathogen identification and drug sensitivity of ascites disease in Rana catesbeiana

A total of 3 bacterial strains were obtained from the sick Rana catesbeiana liver,limb,ascites,and other tissues.Consistency of their physical and chemical properties was confirmed by the observation of colony morphology,Gram staining,physiological and biochemical identification,and they were all Acinetobacter lwoffi.Two different artificial infection ways of abdominal injection and soak confirmed that the bacteria remained pathogenic.Drug sensitive tests showed that the strain W2011-01 was sensitive to 18 kinds of antibiotics,which were Levofloxacin,Meropenem,Piperacillin/Tazobactam,Tobramycin,Trimethoprim/Sulfa,Gentamicin,and so on;and resistant to Nitrofurantoin,Ciprofloxacin,Sarafloxacin,Acetylspiramycin,Penicillin,Bacitracin,Furazolidone,Rifampicin,Ampicillin,and Doxycycline.