低渗溶液浓度对黄颡鱼精子活力和受精率的影响

为提高黄颡鱼(Pelteobagrus fulvidraco)人工授精的稳定性,分别用卵子和精子同步激活法以及预激活精子法,在6个梯度浓度(0%～0.5%)范围的低渗溶液中进行人工授精试验,比较精子活力、受精率和孵化率。结果显示:低渗溶液浓度与精子活力、精子激活率和受精率密切相关,在相同浓度的低渗溶液组中采用预激活精子法的受精率显著高于卵子和精子同步激活法(P<0.05),受精率相对应提高了7.7%～14.5%;低渗溶液的浓度和人工授精方式对孵化率无显著影响。结果表明在浓度0.3%的低渗溶液中采用预激活精子法的效果最好。

Effects of hypotonic solution on the sperm motility and fertilization rate in Yellow catfish

In order to improve the stability of artificial fertilization of Yellow catfish(Pelteobagrus fulvidraco),sperm motility,fertilization rate and hatching rate were evaluated in the experiment of artificial fertilization in 6 levels(ranged from 0% to 0.5%) of hypotonic solution by the methods of synchronous activation of eggs and sperm and pre activation of sperm respectively.The results showed that,hypotonic solution concentration was closely related to sperm motility,sperm activating rate and fertilization rate.In the same concentration of hypotonic solution,fertilization rate by using pre activation of sperm was significantly higher than that by using synchronous activation of eggs and sperm(P<0.05),fertilization rate increased by 7.7%~14.5% accordingly.Hypotonic solution concentration and method of artificial fertilization had no significant impact on hatching rate.The results indicated that pre activation of sperm in 0.3% hypotonic solution had the best effect.