| 超低温冷冻对俄罗斯鲟精浆和精子酶活性的影响 |
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| 引用本文： | 徐滨,江琪,庄平,章龙珍,黄晓荣,王斌,张涛,闫文罡.超低温冷冻对俄罗斯鲟精浆和精子酶活性的影响[J].淡水渔业,2013(1):9-13,54. |
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| 作者姓名： | 徐滨 江琪 庄平 章龙珍 黄晓荣 王斌 张涛 闫文罡 |
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| 作者单位： | 中国水产科学研究院东海水产研究所;中国水产科学研究院长江水产研究所;浙江省杭州千岛湖鲟龙科技开发有限公司;上海海洋大学生命科学与技术学院;上海市高校水产养殖学E-研究院 |
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| 基金项目： | 国家科技基础条件平台(2006DKA30470_004);中央级公益性科研院所基本科研业务费专项资金项目(2007T02);上海市高校水产养殖学E-研究院建设项目(E03009);浙江省科技计划项目(2007C12026);农业科技成果转化基金项目(2008GB23260408) |
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| 摘 要： | 研究了超低温冷冻保存(-196℃)对俄罗斯鲟(Acipenser gueldenstaedti)精浆和精子中总三磷酸腺苷酶(AT-Pase)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)、肌酸激酶(CK)等酶活性的影响,并分别测定了冷冻前后俄罗斯鲟精浆和精子中酶的活性。结果显示,经过超低温冷冻保存后,俄罗斯鲟精子活力下降,精子内各酶活性均显著降低,添加冷冻保护液组精子中总ATPase、SDH、LDH和CK的活性分别从(198.47±14.43)U/mL、(30.00±2.65)U/mL、(6 982.29±24.32)U/L和(1.94±0.05)U/mL下降至(110.19±2.32)U/mL、(16.33±2.08)U/mL、(5 122.93±195.07)U/L和(1.49±0.14)U/mL。未添加抗冻剂组则分别下降至(2.25±0.33)U/mL、(11.67±0.58)U/mL、(4 488.04±78.33)U/L和(1.16±0.02)U/mL;精浆中酶的活性均显著升高,添加冷冻保护液组精浆总ATPase、SDH、LDH和CK活性分别从(12.70±0.57)U/mL、(7.50±0.71)U/mL、(2017.26±116.81)U/L和(2.93±0.59)U/mL升高至(92.49±5.18)U/mL、(13.33±0.58)U/mL、(3 688.97±172.67)U/L和(4.39±0.24)U/mL,未添加抗冻剂组则分别上升至(200.27±12.97)U/mL、(24.67±3.06)U/mL、(6 124.40±329.14)U/L和(5.20±0.16)U/mL。结果表明,超低温冷冻对俄罗斯鲟精浆和精子中酶活性及精子活力均有较大影响。
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| 关 键 词： | 俄罗斯鲟(Acipenser gueldenstaedti) 精子 超低温冷冻 酶活性 |
Effects of cryopreservation on the activities of enzyme activities of seminal plasma semen in Acipenser gueldenstaedti |
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| XU Bin,JIANG Qi,ZHUANG Ping,ZHANG Long-zhen,HUANG Xiao-rong,WANG Bin,ZHANG Tao,YAN Wen-gang.Effects of cryopreservation on the activities of enzyme activities of seminal plasma semen in Acipenser gueldenstaedti[J].Freshwater Fisheries,2013(1):9-13,54. |
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| Authors: | XU Bin JIANG Qi ZHUANG Ping ZHANG Long-zhen HUANG Xiao-rong WANG Bin ZHANG Tao YAN Wen-gang |
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| Institution: | 1(1.East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China; 2.Yangtz River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China; 3.College of Aqua-life Science and Technology,Shanghai Ocean University,Shanghai 200090,China; 4.Aquaculture Division,E-Institute of shanghai Universites,Shanghai 200090,China; 5.Hangzhou Qiandaohu Xunlong Technology Development Co.,Ltd,Hangzhou 311700,China) |
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| Abstract: | The effects of cryopreservation(-196 ℃) on were studied,and the activities of enzyme in the enzyme′s activities(total ATPase,SDH,LDH and CK) in seminal plasma and spermatozoa of Acipenser gueldenstaedti.The changes of enzyme′s activities were the seminal plasma and spermatozoa were determined hefore and after cryopreservation.The results showed that the motility of spermatozoa dropped after cryopreservation,the activities of enzyme in spermatozoa descended significantly(P<0.05),and the activities of total ATPase,SDH,LDH,CK in spermatozoa in cryoprotectant group dropped from(198.47±14.43)U/mL,(30.00±2.65)U/mL,(6982.29±24.32)U/L and(1.94±0.05)U/mL to(110.19±2.32)U/mL,(16.33±2.08)U/mL,(5122.93±195.07)U/L and(1.49±0.14)U/mL,respectively.The activities of enzyme in spermatozoa without cryoprotectant dropped to(2.25±0.33)U/mL,(11.67±0.58)U/mL,(4488.04±78.33)U/L and(1.16±0.02)U/mL,the activities of enzyme in seminal plasma increased significantly(P<0.05),the activities of total ATPase,SDH,LDH,CK in seminal plasma in cryoprotectant group rose from(12.70±0.57)U/mL,(7.50±0.71)U/mL,(2017.26±116.81)U/L and(2.93±0.59)U/mL to(92.49±5.18)U/mL,(13.33±0.58)U/ml,(3688.97±172.67)U/L and(4.39±0.24)U/mL,respectively.The activities of enzyme in seminal plasma without cryoprotectant rose to(200.27±12.97)U/mL,(24.67±3.06)U/mL,(6124.40±329.14)U/L and(5.20±0.16)U/mL.It was indicated that the activities of enzyme and the motility of spermatozoa of A.gueldenstaedti were greatly offected by cryopreservation. |
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| Keywords: | Acipenser gueldenstaedti spermatozoa cryopreservation enzyme′s activities |
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