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浙江省桃花水母18S rRNA基因序列分析及物种鉴定
引用本文:徐善良,周剑君,王丹丽,孙雪.浙江省桃花水母18S rRNA基因序列分析及物种鉴定[J].淡水渔业,2010,40(2).
作者姓名:徐善良  周剑君  王丹丽  孙雪
作者单位:1. 宁波大学应用海洋生物技术教育部重点实验室,浙江宁波,315211;宁波大学海洋生物工程浙江省重点实验室,浙江宁波,315211
2. 宁波大学应用海洋生物技术教育部重点实验室,浙江宁波,315211
3. 宁波大学海洋生物工程浙江省重点实验室,浙江宁波,315211
基金项目:教育部长江学者与创新团队项目,浙江省自然科学基金 
摘    要:采用PCR和DNA测序技术扩增和测序了2003~2008年间采自浙江省宁波、杭州、温州和金华4个地区7个地点桃花水母(Craspedacusta)核糖体小亚基rRNA(18S rRNA)基因,并与已知索氏桃花水母(Craspedacusta sow-erbyi)和中华桃花水母(Craspedacusta sinensis)18S rRNA基因序列进行了比对。结果显示:这7个地点的桃花水母与索氏桃花水母序列极为相似,相似度达99.67%~100%,遗传距离为0~0.002,而与同属中华桃花水母的遗传距离为0.013~0.015。分子系统学分析显示这7个地点桃花水母与索氏桃花水母分支的置信度高达100%。结果确定分析样品均为索氏桃花水母。

关 键 词:索氏桃花水母(Craspedacusta  sowerby)  18SrRNA  分类学

Sequence analysis of 18S rRNA gene of Craspedacusta sowerbyi in Zhejiang province
XU Shan-liang,ZHOU Jian-jun,WANG Dan-li,SUN Xue.Sequence analysis of 18S rRNA gene of Craspedacusta sowerbyi in Zhejiang province[J].Freshwater Fisheries,2010,40(2).
Authors:XU Shan-liang  ZHOU Jian-jun  WANG Dan-li  SUN Xue
Institution:XU Shan-liang1,2,ZHOU Jian-jun1,WANG Dan-li1,SUN Xue2(1.Key Laboratory of Applied Marine Biotechnology,Ministry of Education,Ningbo University,Ningbo,Zhejiang 315211,2.Key Laboratory of Marine Biotechnology of Zhejiang,Zhejiang 315211)
Abstract:Craspedacusta sp.samples were collected at 7 locations from 4 region of Zhejiang Province including Ningbo,Hangzhou,Jinhua and Wenzhou during from 2003 to 2008.In order to further determine the accuracy of their classification status,the small subunit ribosomal RNA gene(18S rRNA)of Craspedacusta sp.from the 7 locations was amplified and sequenced,and compared to those of Craspedacusta sowerbyi and Craspedacusta sinensis.The result showed that the 18S rRNA of Craspedacusta sp.is highly similar to that of C.s...
Keywords:18S rRNA
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