罗非鱼性别相关微卫星标记的初步筛选

为分析罗非鱼群体的遗传多样性以及筛选与罗非鱼性别相关的微卫星标记。应用24对微卫星引物,使用常规PCR及聚丙烯酰胺凝胶电泳的方法在两个尼罗罗非鱼(Oreochromis niloticus)群体和两个奥利亚罗非鱼(O.aureus)群体中初步筛选到UNH931、GM128、GM201、GM258、GM597以及UNH898共6个与性别相关的微卫星标记。然后使用降落PCR以及毛细管电泳的方法在两个尼罗罗非鱼群体、两个奥利亚罗非鱼群体以及ZY 1、WY 1、YY 1和YY 2型罗非鱼群体中进一步扩增这6个微卫星标记,统计各群体的遗传多样性参数:6个微卫星标记在上述群体共297个样本中检测到95个等位基因,其大小在97~302 bp之间,各位点在各群体等位基因数在1~13个之间,各群体平均观测等位基因数为2.167~9.333,平均有效等位基因数为1.624~4.966,平均观测杂合度为0.324~0.983,平均期望杂合度为0.329~0.782,平均多态信息含量为0.275~0.753;两个尼罗罗非鱼群体、WY 1和YY 2群体达到高度多态(PIC>0.5),两个奥利亚罗非鱼群体、ZY 1和YY 1群体为中度多态(0.25

Preliminary screening of sex-related microsatellite markers in Oreochromis niloticus and O.aureus

A total of 24 pairs of microsatellite primers were used to screen sex-related microsatellite markers in two Oreochromis niloticus populations and two O.aureus populations by conventional PCR and polyacrylamide gel electrophoresis to analyze the genetic diversity of tilapia and to screen microsatellite markers related to tilapia sex.Six microsatellite markers,including UNH931,GM128,GM201,GM258,GM597 and UNH898 were identified related to tilapia sex.These six microsatellite markers were then amplified in the two O.niloticus populations,two O.aureus populations and the ZY 1,WY 1,YY 1,YY 2 tilapia populations using touchdown PCR and capillary electrophoresis.The genetic diversity parameters of each population were counted at the same time.A total of 95 alleles in 297 samples from the above populations were detected by six microsatellite markers,ranging in size from 97 to 302 bp.The number of alleles in each population ranged from 1 to 13,the average number of observed alleles was 2.167-9.333,the average effective allele number was 1.624-4.966,the average observed heterozygosity was 0.324-0.983,the average expected heterozygosity was 0.329-0.782,and the average polymorphic information content was 0.275-0.753.The two O.niloticus populations of WY 1 and YY 2 reached a high polymorphism(PIC>0.5),and the two O.aureus populations of ZY 1 and YY 1 were moderately polymorphic(0.25