怀山药皮对淇河鲫血清生化、免疫指标及组织结构的影响

为探究怀山药皮(Chinese yam peel,YP)对淇河鲫免疫的影响,选取平均体重为(106.76±3.74)g的淇河鲫240尾,随机分为对照组(NC)、0.5%怀山药皮添加组(LYP)、1%怀山药皮添加组(MYP)、2%怀山药皮添加组(HYP)4组,养殖8周,检测实验鱼生长指标、血清生化指标及免疫相关基因的表达情况。结果显示:添加怀山药皮对淇河鲫生长指标没有显著性影响;血液生化指标中谷草转氨酶活性在MYP组显著性降低,碱性磷酸酶的活性显著升高;与NC组相比,怀山药皮添加组肝脏细胞膜与核膜完整性增强,胞质破碎,核溶解、偏移现象减轻;与NC组相比,添加怀山药皮上调了抑炎因子(IL-10)、紧密连接蛋白基因(Claudin-1)、抗氧化基因(CAT和GST)和TLR通路基因(TLR4)mRNA表达量。以上结果表明,饲料中添加怀山药皮,能在一定程度上增强鱼体免疫力。

The influence of the Chinese yam peel on serum biochemistry,immunity indexes and tissue structure of Carassius auratus

A total of 240 Qihe crucian carp(Carassius auratus)with an average weight of(106.76±3.74)g were randomly divided into four groups to explore the effect of Chinese yam peel(YP)on the immunity,.The four groups were NC group(0%yam peel addition),LYP group(0.5%yam peel addition),MYP group(1%yam peel addition)and HYP group(2%yam peel addition).After feeding for 8 weeks,the growth indexes,serum biochemical indexes and immune-related gene expressions of the experimental fish were detected.The results showed that there was no significant effect on the growth index of Qihe crucian carp.Blood biochemical indexes showed that the content of glutamate transaminase was significantly decreased in MYP group.Also,the activity of alkaline phosphatase was significantly increased in MYP.Based on the HE staining,the liver cell membrane,nuclear integrity,while the karyolysis migration rate was reduced.Gene expression results showed that the addition of Chinese yam peel increased inflammation suppression factor(IL-10),tight junction protein gene(Claudin-1),antioxidant gene(CAT and GST)and the pathways gene(TLR4)mRNA expression.As a conclusion,Chinese yam peel could improve the immunity of the Qihe crucian carp.