| 平胸龟遗传多样性的RAPD分析 |
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| 引用本文： | 马丽莎,郑光明,朱新平,刘毅辉,陈永乐,罗建仁.平胸龟遗传多样性的RAPD分析[J].淡水渔业,2007,37(2):76-79. |
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| 作者姓名： | 马丽莎 郑光明 朱新平 刘毅辉 陈永乐 罗建仁 |
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| 作者单位： | 1. 中国水产科学研究院珠江水产研究所,广州,510380;广东海洋大学,广东湛江,524025
2. 中国水产科学研究院珠江水产研究所,广州,510380 |
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| 摘 要： | 应用RAPD技术对平胸龟(Platysternon megacephalum Gay)的遗传多样性进行了分析,用27个随机引物对福建寿宁平胸龟21个个体的基因组DNA进行了PCR扩增,共扩增出5187条DNA片段,平均每个个体扩增出247条带。在检测到的247条带中,多态性条带为141条,多态性条带百分比为55.97%(25.0%~88.89%),条带大小在100bp~2000bp之间,21个个体间遗传距离为0.0972~0.3198,平均遗传距离为0.1750±0.0387,表明平胸龟具有较高的遗传多样性水平。采用类平均聚类法(NJTREE)构建了21个个体相互关系的分子聚类图,表明该地区平胸龟并没有形成种群的分化。
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| 关 键 词： | 平胸龟(Platysternon megacephalum Gay) 遗传多样性 随机扩增多样性分析 |
| 文章编号： | 1000-6907-(2007)02-0076-04 |
| 修稿时间： | 2006-12-14 |
Genetic Diversity Analysis of Platysternon megacephalum by RAPD Method |
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| MA Li-sha,ZHENG Guang-ming,ZHU Xin-ping,LIU Yi-hui,CHENG Yong-le,LUO Jian-ren.Genetic Diversity Analysis of Platysternon megacephalum by RAPD Method[J].Freshwater Fisheries,2007,37(2):76-79. |
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| Authors: | MA Li-sha ZHENG Guang-ming ZHU Xin-ping LIU Yi-hui CHENG Yong-le LUO Jian-ren |
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| Institution: | 1.Pearl River Fisheries Research Institute, Chinese Academy Fishery of Sciences, Guangzhou 510380;2. Guangdong Ocean University, Zhanjiang , Guangdong 524025 |
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| Abstract: | Genetic diversity of 21 individuals of Platystern on megacephalum(Gay)was analyzed by RAPD with 27 10 -nuclectied-long primers,the total of 5187 fragments were amplified,averaged 2 47 bands in each individual,141 of 247 loci were polymorphic,amounting to 55.97 %,the size of DNA fragments ranged from 100bp to 2000bp.The genetic distance bet ween two individuals was from 0.0972 to 0.3198.The average genetic distance wa s 0.1750± 0.0387.The phylogenetic tree of the 21 individuals was obtained by NJTREE analysis in the RAPDistance 1.04 program,it showed that the high genetic diversity existed in the turtle. |
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| Keywords: | Platysternon megacephalum Genetic diversity RAPD analysis |
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