| 4个群体鲢mtDNA D-loop的PCR-RFLP分析 |
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| 引用本文： | 王淞,曹晓霞,谷口顺彦,董仕.4个群体鲢mtDNA D-loop的PCR-RFLP分析[J].淡水渔业,2010,40(4). |
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| 作者姓名： | 王淞 曹晓霞 谷口顺彦 董仕 |
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| 作者单位： | 1. 天津师范大学生命科学学院,细胞遗传与分子调控天津市重点实验室,天津,300387
2. 福山大学生命工学部,福山市,日本,722-2101 |
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| 基金项目： | 国家自然科学基金资助项目，天津师范大学校内基金 |
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| 摘 要： | 采用PCR技术扩增了湖北监利、江西瑞昌、湖南长沙3个长江野生群体和天津宁河1个人工繁殖群体(已繁殖6代)共158尾鲢(Hypophthalmichthys molitrix)mtDNA D-loop区段,并用14种核酸内切限制酶对扩增片段进行酶切。结果显示:所有试验鱼均扩增出约1.6 kb的DNA片段,10种限制酶有酶切位点,共检测出16种单倍型,以单倍型Ⅰ为主,在各群体中所占比例为57.5%~90.0%。鲢4个群体的单倍型多样性指数、核苷酸多样性指数为0.1885~0.6526和0.001182~0.007570。瑞昌群体与监利群体的Rogers遗传距最小,为0.1250;监利群体与宁河群体的Rogers遗传距最大,为0.2693。χ2检验结果显示4个群体间的遗传差异显著(P<0.01)。在4个群体中,宁河人工繁殖群体的单倍型多样性指数、核苷酸多样性指数均最高,并且HinfⅠ的C酶切类型为宁河群体所特有,达20.0%。
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| 关 键 词： | 鲢(Hypophthalmichthys molitrix) mtDNAD-loop PCR-RFLP 遗传多样性 Rogers遗传距 |
PCR-RFLP analysis on mtDNA D-loop region of four populations of silver carp (Hypophthalmichthys molitrix) |
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| WANG Song,CAO Xiao-xia,TANIGUCHI Nobuhiko,DONG Shi.PCR-RFLP analysis on mtDNA D-loop region of four populations of silver carp (Hypophthalmichthys molitrix)[J].Freshwater Fisheries,2010,40(4). |
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| Authors: | WANG Song CAO Xiao-xia TANIGUCHI Nobuhiko DONG Shi |
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| Institution: | WANG Song1,CAO Xiao-xia1,TANIGUCHI Nobuhiko2,DONG Shi1(1.College of Life Science,Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin Normal University,Tianjin 300387,2.Faculty of Life Science and Biotechnology,Fukuyama University,Fukuyama,Japan 722-2101) |
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| Abstract: | The restriction fragment length polymorphisms of the mtDNA D-loop region of 158 individuals of silver carp(Hypophthalmichthys molitrix) were analyzed with PCR technique.The wild samples were collected from Yangtze River drainage area(Jianli section in Hubei Province,Ruichang section in Jiangxi Province and Changsha section in Hunan Province) respectively.The artificially propagated population,the sixth generation,originated from Yangtze River 1957,were collected from hatchery station in Ninghe area,Tianjin ... |
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| Keywords: | mtDNA D-loop PCR-RFLP |
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