| 三峡库区3种银鱼线粒体DNA细胞色素b基因序列多态性分析 |
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| 引用本文： | 罗宏伟,段辛斌,王珂,唐锡良,陈大庆.三峡库区3种银鱼线粒体DNA细胞色素b基因序列多态性分析[J].淡水渔业,2009,39(6). |
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| 作者姓名： | 罗宏伟 段辛斌 王珂 唐锡良 陈大庆 |
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| 作者单位： | 1. 西南大学生命科学学院,重庆北碚,400715;农业部长江上游渔业资源环境重点野外科学观测试验站,中国水产科学研究院长江水产研究所,湖北荆州,434000
2. 农业部长江上游渔业资源环境重点野外科学观测试验站,中国水产科学研究院长江水产研究所,湖北荆州,434000 |
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| 基金项目： | 国家科技支撑计划，国家自然科学基金 |
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| 摘 要： | 对三峡库区大银鱼(Protosalanx chinensis)、太湖新银鱼(Neosalanx taihuensis)和短吻间银鱼(Hem isalanxbrachyrostralis)共78尾个体的Cyt b基因全序列(1141bp)进行了分析。结果显示,序列有16个变异位点,总变异率为1.4%,其中13个为简约位点。大银鱼的32尾样本有6个单倍型,太湖新银鱼32尾样本有5个单倍型,短吻间银鱼14尾样本中有3个单倍型。大银鱼、太湖新银鱼和短吻间银鱼的单倍型多样性指数(h)分别为0.804±0.032,0.671±0.061,0.385±0.149;核苷酸多样性指数(π)分别为0.00146±0.00009,0.00228±0.00024,0.00067±0.00026。3种银鱼群体的遗传距离在0.12461~0.23796之间,净遗传距离在0.12274~0.24418之间。基于Cyt b基因序列,利用K imura-2模型构建的NJ树表明:大银鱼与太湖新银鱼种群的亲缘关系最近,两者与短吻间银鱼种群的亲缘关系较远。研究结果表明,三峡库区3种银鱼的遗传多样性低。
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| 关 键 词： | 大银鱼(Protosalanx chinensis) 太湖新银鱼(Neosalanx taihuensis) 短吻间银鱼(Hemisalanx brachyrostralis) 线粒体DNA 细胞色素b基因 遗传多样性 三峡库区 |
Genetic polymorphism analysis of mitochondrial DNA cytochrome b sequences of three species of salangids in the Three Gorges Reservior |
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| LUO Hong-wei,DUAN Xin-bing,WANG Ke,TANG Xi-liang,CHEN Da-qing.Genetic polymorphism analysis of mitochondrial DNA cytochrome b sequences of three species of salangids in the Three Gorges Reservior[J].Freshwater Fisheries,2009,39(6). |
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| Authors: | LUO Hong-wei DUAN Xin-bing WANG Ke TANG Xi-liang CHEN Da-qing |
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| Institution: | LUO Hong-wei1,2,DUAN Xin-bing2,WANG Ke2,TANG Xi-liang1,CHEN Da-qing2(1.College of Life Science,Southwest University,Beibei,Chongqing 400715,2.Fishery Resource and Key Field Station of Environment in Upper-middle Reaches of Yangtze River,Ministry of Agriculture,Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Jingzhou,Hubei 434000) |
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| Abstract: | In this study,the genetic polymorphism of mitochondrial DNA cytochrome b sequences of three species of salangids(Protosalanx chinensis,Neosalanx taihuensis and Hemisalanx brachyrostralis) in the Three Gorges Reservior was analyzed to understand the source and the genetic diversity.The complete mtDNA cytochrome b gene sequence(1141bp) of 78 individuals were obtained from P.chinensis,N.taihuensis and H.brachyrostralis in the Three Gorges Reservior.16 variable sites were detected,accounting for 1.4% of total s... |
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| Keywords: | Protosalanx chinensis Neosalanx taihuensis Hemisalanx brachyrostralis mitochondrial DNA cytochrome b gene genetic diversity Three Gorges Reservior |
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