饲料中添加复合芽孢杆菌对草鱼消化道酶活性及肠道菌群的影响

用含复合芽孢杆菌(105cfu/g饲料,枯草芽孢杆菌和解淀粉芽孢杆菌以1∶1比例混合)的基础日粮,按体重3%的日投饵量,饲喂体重(51.37±0.58)g的草鱼(Ctenopharyngodon idellus),饲养实验45 d。结果表明:与对照组相比,处理组肠道内容物胰蛋白酶活性提高了79.46%(P<0.01);肝胰腺脂肪酶活性提高了16.82%(P<0.01),肝胰腺淀粉酶和胰蛋白酶活性无显著差异(P>0.05)。肠道菌群数量分析显示,处理组肠道芽孢杆菌数均显著提高(P<0.01),弧菌和大肠杆菌数显著降低(P<0.01)。结果提示,饵料中添加复合芽孢杆菌能够改善草鱼肠道菌群组成,并提高消化道特定消化酶活性。

Effects of dietary Bacillus preparation on digestive enzyme activity and intestinal microflora in Ctenopharyngodon idellus

This experiment was conducted to study the effects of dietary Bacillus preparation on digestive enzyme activity and intestinal microflora in Ctenopharyngodon idellus.C.idellus,weighing(51.37±0.58)g,were randomly divided into 2 treatments with 3 replicates and 50 fish for each tank.The control group was fed with basal diet,and treatment group diet was added with 0.1% Bacillus complex(Bacillus subtilis and Bacillus amyloliquefaciens,1∶1).Two groups were fed with feed about 3% fish weight.The feeding trail lasted for 45 days.The results showed that 1.In intestinal,trypsin activity of treatment group increased by 79.46%(P<0.01);2.In hepatopancreas,lipase activity of treatment group increased by 16.82%(P<0.01),but the trypsin and amylase activities were not significantly affected(P>0.05);3.Analysis of intestinal microflora showed that,the number of Bacillus sp.in the intestine of treatment group increased significantly(P<0.01),while the number of E.coli and vibrios decreased significantly(P<0.01).These results indicated that supplementation of Bacillus preparation can increase the digestive enzyme activities in alimentary tract and improve the intestinal microflora composition of C.idellus.