流式细胞术在克氏原螯虾血细胞的分类、活性和免疫功能研究中的应用

应用流式细胞术(FCM)对克氏原螯虾(Procambarus clarkii)血细胞的分类、活性和免疫功能进行了研究。结果显示:血细胞可分为透明细胞、小颗粒细胞和大颗粒细胞三个亚群,组成比例分别为(26.25±5.29)%、(51.44±7.02)%和(11.20±1.82)%;螯虾血细胞的平均总凋亡率约为3.12%;血细胞对荧光大肠杆菌的吞噬活力显著(P<0.05)高于荧光微球,吞噬率分别为17.04%和14.57%;血细胞在自然生理状态下含有一定量的活性氧,其在两类颗粒细胞的含量显著(P<0.05)高于透明细胞,在大颗粒细胞中最高。结果表明,FCM能较好地应用于虾类的血细胞分类和功能研究。

Application of flow cytometry in studies of classification, viability and immune parameters of Procambarus clarkii Haemocytes

Classification,viability and immune function of crayfish(Procambarus clarkii) haemocytes were.studiedusing flow cytometry.Results showed that haemocytes were divided into three subpopulations,including hyaline cells,semigranular cells and granular cells,with the proportions of(26.25±5.29)%,(51.44±7.02)% and(11.20±1.82)%,respectively.The average apoptotic ratio was 3.12% in the total haemocytes.Phagocytic activity against fluorescent E.coli was significantly higher than that of fluorescent microsphere(P<0.05).The phagocytic ratios were 17.04 and 14.57%,respectively.Haemocytes also produced reactive oxygen species at the natural physiological state.The reactive oxygen production was significantly higher in the two granular cells than hyaline cells(P<0.05),and highest in granular cells.The present study showed the superiority of FCM on study of cellular classification and functions of shrimp haemocytes.