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| 患病黄额闭壳龟中产酸克雷伯菌的分离与鉴定 | |
| 引用本文： | 周勇,曾令兵,李瑞伟,高正勇,孙建滨,范玉顶.患病黄额闭壳龟中产酸克雷伯菌的分离与鉴定[J].淡水渔业,2012,42(3):38-43. |
| 作者姓名： | 周勇 曾令兵 李瑞伟 高正勇 孙建滨 范玉顶 |
| 作者单位： | 1. 中国水产科学研究院长江水产研究所,武汉,430223 2. 中国水产科学研究院长江水产研究所,武汉430223;上海海洋大学水产与生命学院,上海201306;华中农业大学水产学院,武汉430070 3. 上海海洋大学水产与生命学院,上海,201306 4. 华中农业大学水产学院,武汉,430070 |
| 基金项目： | 公益性行业(农业)科研专项 |
| 摘 要： | 从患病黄额闭壳龟(Cuora galbinifrons)肝脏中分离到一株致病菌HE01,经人工感染健康巴西龟,可复制与自然发病相同的症状,且从人工感染病龟体内再次分离到相同的病原菌。经Biolog微生物自动鉴定系统的鉴定,以及进一步的16 S rDNA基因序列和系统发育分析都表明,此致病菌为产酸克雷伯菌(Klebsiella oxytoca)。药物敏感性试验表明,该菌株对头孢噻肟、头孢曲松、头孢哌酮等10种药物高度敏感。 |
| 关 键 词： | 黄额闭壳龟(Cuora galbinifrons) 产酸克雷伯菌(Klebsiella oxytoca) 分离鉴定 药敏试验 |
Isolation and identification of Klebsiella oxytoca from diseased Cuora galbinifrons |
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| ZHOU Yong , ZENG Ling-bing , LI Rui-wei , GAO Zheng-yong , SUN Jian-bin , FAN Yu-ding.Isolation and identification of Klebsiella oxytoca from diseased Cuora galbinifrons[J].Freshwater Fisheries,2012,42(3):38-43. | |
| Authors: | ZHOU Yong ZENG Ling-bing LI Rui-wei GAO Zheng-yong SUN Jian-bin FAN Yu-ding |
| Institution: | 1(1.Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences,Wuhan 430223,China; 2.College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China; 3.College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China) |
| Abstract: | A bacterial strain HE01 was isolated and identified from diseased Cuora galbinifron.Infection with the bacterial suspension to healthy red-ear slider could reproduce the diseased symptoms as occurred naturally and the same bacterium could be recovered from these infected C.galbinifron.Identification by the Biolog Microbial Identification System,and further 16 S rDNA sequence and phylogenetic analysis demonstrated that the bacterium isolated from diseased C.galbinifron was Klebsiella oxytoca.The susceptibility test to antibiotics demonstrated that the bacterial strain HE01 was highly susceptible to cefotamine,ceftriaxone,cefobid,and so on. |
| Keywords: | Cuora galbinifrons Klebsiella oxytoca isolation and identification antibiotic susceptibility test |
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