盐酸诺氟沙星在奥尼罗非鱼体内的残留消除规律

用高效液相色谱法(HPLC)研究盐酸诺氟沙星在奥尼罗非鱼(Oreochromis aureus×O.niloticus)体内残留消除规律。结果显示:此方法回收率为73.08%~89.61%,最低检测限为0.2 ng/mL;灌药第5天,4种组织药物含量达到最高,随后迅速下降;药物残留消除速率为:血液>肌肉>肝脏>肾脏。结果表明:在(23±1)℃水温条件下,盐酸诺氟沙星在罗非鱼肌肉中降到50μg/kg的休药期为13 d,在罗非鱼内脏中降到50μg/kg的休药期为22 d。

Residue Elimination of Norfloxacin Hydrochloride in Hybrid Tilapia ( Oreochromis aureus ×O. niloticus )

Tissue residue elimination of norfloxacin hydrochloride was evaluated in hybrid tilapia(Oreochromis aureus×O.niloticus).Norfloxacin hydrochloride concentrations were determined by means of high performance liquid chromatography(HPLC)with 0.2 ng/mL detection limit,and the overall recoveries were 73.08%～89.61%.Results of this study showed that norfloxacin hydrochloride residues gradually accumulated in four tissues during dosing time and the highest level was observed on the 5th dosing day(the last dosing time).The elimination rates were different in the four tissues and the order was serum>muscle>liver>kidney.According to the maximum residues limit(MRL)of 50 μg/kg body weight in edible tissue or viscera,the withdrawal time should be at least 13 days or 22 days prior to the use of dosed tilapia as human food following multi-oral administrations at water temperature of(23±1)℃.