| 养殖澳洲宝石鱼迟缓爱德华氏菌的分离鉴定及致病基因的检测 |
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| 引用本文： | 叶旭红,林先贵,王一明.养殖澳洲宝石鱼迟缓爱德华氏菌的分离鉴定及致病基因的检测[J].淡水渔业,2010,40(1). |
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| 作者姓名： | 叶旭红 林先贵 王一明 |
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| 作者单位： | 1. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京,210008;中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,南京,210008;中国科学院研究生院,北京,100049
2. 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,南京,210008;中国科学院南京土壤研究所-香港浸会大学土壤与环境联合开放实验室,南京,210008 |
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| 基金项目： | 国家自然科学基金(20607024);;国家“十一五”科技支撑计划(2006BAD10B05);;农业科技成果转化资金项目(2006C40038) |
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| 摘 要： | 从患病澳洲宝石鱼体内分离到一株致病菌(编号Et4),对该菌的生化特性及致病基因进行检测,并进行了药敏和人工感染实验。结果显示:菌株Et4的生化鉴定结果与迟缓爱德华氏菌(Edwardsiella tarda)标准菌株(AY77513)一致;其16S rRNA序列,经同源性比对与迟缓爱德华氏菌核苷酸相似度最高,达99%;根据迟缓爱德华氏菌的致病基因Ⅲ型分泌系统装置蛋白esaV基因序列设计引物,进行PCR扩增,得到708 bp序列,该序列与迟缓爱德华氏菌的esaV基因序列相似性达99.3%,说明菌株Et4具有esaV基因。菌株Et4对环丙沙星等较敏感,对其它药物中度或不敏感,具有较强的致病力(LD50=3.74×104CFU/mL),从病鱼中可以重新分离出此菌。综合形态学、生化特性、16S rDNA序列及致病基因序列鉴定其为迟缓爱德华氏菌。
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| 关 键 词： | 澳洲宝石鱼(Scortum barcoo) 迟缓爱德华氏菌(Edwardsiella tarda) 16S rRNA基因 esaV基因 |
Identification and detection of virulence gene of the pathogenic bacteria Edwardsiella tarda in cultured Scortum barcoo |
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| YE Xu-hong,LIN Xian-gui,WANG Yi-ming.Identification and detection of virulence gene of the pathogenic bacteria Edwardsiella tarda in cultured Scortum barcoo[J].Freshwater Fisheries,2010,40(1). |
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| Authors: | YE Xu-hong LIN Xian-gui WANG Yi-ming |
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| Institution: | 1.State Key Laboratory of Soil and Sustainable Agriculture;Institute of Soil Science;Chinese Academy of Sciences;Nanjing 210008;2.Joint Open Laboratory of Soil and the Environment;Chinese Academy of Sciences and Hongkong Baptist University;3.Graduate University of Chinese Academy of Sciences;Beijing 100049 |
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| Abstract: | Bacterial strain Et4 was isolated from diseased Jade perch(Scortum barcoo).The biochemical characteristics of the strain Et4 and the virulence gene were detected,and carried out sensitivity and challenge tests.The results indicated that:the strain Et4 was Edwardsiella tarda with 99% reliability resulted by API ID32E.The 16S rRNA gene sequence analysis revealed that the strain Et4 exhibited the highest level of similarity of 99% identity with E.tarda compared with GenBank database.Thus,the strain Et4 was ide... |
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| Keywords: | Scortum barcoo Edwardsiella tarda 16S rRNA gene esaV gene |
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