盐酸诺氟沙星在奥尼罗非鱼体内的药动学研究

按照鱼的体重,以10 mg/kg的单剂量,分别给奥尼罗非鱼(Oreochromis aureus×O.niloticus)水剂口灌和混饲口灌盐酸诺氟沙星,用高效液相色谱法(HPLC)检测给药后各个时间点的血药浓度。结果显示:水剂口灌组和混饲口灌组的药时数据符合开放性二室模型,水剂口灌组药物的吸收、消除都明显快于混饲组,水剂口灌组主要药动学参数为:t1/2ka=0.269 h,t1/2α=0.588 h,t1/2β=16.42 h,Tmax=0.745 h,Cmax=0.123μg/mL,AUC=1.83 h;混饲口灌组主要药代动力学参数:t1/2ka=0.428 h,t1/2α=1.85 h,t1/2β=21.77 h,Tmax=1.63 h,Cmax=0.099μg/mL,AUC=2.42 h。

Study on Pharmacokinetics of Norfloxacin Hydrochloride in Hybrid Tilapia(Oreochromis aureus×O.niloticus)

Hybrid tilapias were treated by oral administration of norfloxacin hydrochloride in either aqueous solution or medicated feed in a single dose of 10mg/kg body weight.The concentration of hybrid tilapia plasma was determined by high performance liquid chromatography,and pharmacokinetic analysis was performed by the computer program 3P97(version 1.0,edited by the Chinese society of mathematical pharmacology,P.R.China).The data of pharmacokinetics conformed to a two-compartment model with first-order absorption after oral administration of norfloxacin hydrochloride in aqueous solution,the main parameters were as follows:t1/2ka=0.269 h,t1/2α= 0.588 h,t1/2β= 16.42 h,Tmax =0.745 h,Cmax= 0.123 μg/mL,AUC=1.83 h.The data of pharmacokinetics also conformed to a two-compartment model with first-order absorption after oral administration of norfloxacin hydrochloride in medicated feed,the main parameters were followed:t1/2ka=0.428 h,t1/2α= 1.85 h,t1/2β= 21.77 h,Tmax=1.63 h,Cmax=0.099 μg/mL,AUC=2.42 h.The results showed that the aqueous solution group was much more quickly than the medicated feed group at drug absorption and elimination.