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金鱼致病菌的分离鉴定与药敏特性
引用本文:曹海鹏,刘丽玲,李怡,杨先乐.金鱼致病菌的分离鉴定与药敏特性[J].淡水渔业,2010,40(6).
作者姓名:曹海鹏  刘丽玲  李怡  杨先乐
基金项目:现代农业产业技术体系建设专项资金,农业部水产种质资源与利用重点开放实验室专项,上海高校选拔培养优秀青年教师科研专项,淡水鱼类出血性疾病综合防治技术集成示范
摘    要:采用常规方法对患病金鱼(Carassius auratus)内脏组织(肝脏、肾脏、性腺)中致病菌进行了分离鉴定与药敏特性研究。结果显示:实验共分离了8株优势菌,经人工回归感染实验证实其中3株细菌(菌株LK-T、PL-R和PK-T)具有致病性。ATB细菌鉴定仪鉴定结果显示,菌株LK-T和菌株PL-R为温和气单胞菌(Aeromonas so-bria),菌株PK-T为豚鼠气单胞菌(Aeromonas punctata subsp.caviae)。这3株致病菌的主要特征为:革兰氏染色阴性,杆状,对奈替米星、头孢呋肟、头孢拉啶、庆大霉素、环丙沙星、新霉素、先锋必等药物均高度敏感。其中庆大霉素、环丙沙星、新霉素等常规渔药可作为防治用药的依据。

关 键 词:金鱼(Carassius  auratus)  温和气单胞菌(Aeromonas  sobria)  豚鼠气单胞菌(Aeromonas  punctata  subsp.caviae)  药敏特性

Isolation,identification and drug sensitivity of pathogen from goldfish
CAO Hai-peng,LIU Li-ling,LI Yi,YANG Xian-le.Isolation,identification and drug sensitivity of pathogen from goldfish[J].Freshwater Fisheries,2010,40(6).
Authors:CAO Hai-peng  LIU Li-ling  LI Yi  YANG Xian-le
Abstract:Eight dominant bacteria were isolated from the abodominal organs(liver,kidney and gonad)of the diseased goldfish using the conventional method.Among the eight strains,three bateria,temporarily named LK-T,PL-R and PK-T,were proved to be pathogenic,and were identified by ATB bacteria identification instrument.The results showed that strain LK-T and PL-R were Aeromonas sobria,strain PK-T was Aeromonas punctata subsp.caviae.The three pathogenic bacteria were all gram-negative and rod-shaped.They were highly sensitive to netilmicin,cefuroxime,cefradine,gentamicin,ciprofloxacin,neomycin and cefobid.Therefore,gentamicin,ciprofloxacin,and neomycin could be used for the control of this disease.
Keywords:Carassius auratus  Aeromonas sobria  Aeromonas punctata subsp  caviae  drug sensitivity
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