| 鲫胰岛素样生长因子-Ⅰ cDNA克隆及序列分析 |
| |
| 引用本文： | 刘军,陈爱敬,黄峰,赵胜军,胡兵,侯永清.鲫胰岛素样生长因子-Ⅰ cDNA克隆及序列分析[J].淡水渔业,2007,37(4):24-29. |
| |
| 作者姓名： | 刘军 陈爱敬 黄峰 赵胜军 胡兵 侯永清 |
| |
| 作者单位： | 武汉工业学院动物营养与饲料科学湖北省重点实验室,武汉,430023 |
| |
| 摘 要： | 利用One-Step RT-PCR技术,从鲫(Carassius auratus)肝胰脏组织中克隆了胰岛素样生长因子-Ⅰ(IGF-Ⅰ),并进行了序列分析。结果表明,鲫IGF-ⅠcDNA由486个核苷酸组成,编码包括信号肽、成熟肽(B、C、A、D四个域)和E域的161个氨基酸。核苷酸同源性分析揭示,鲫IGF-ⅠcDNA序列与金鱼的同源性最高,为99.18%;与鲮、三角鲂、团头鲂的同源性次之,分别为94.44%、94.65%、94.44%;与鲤、草鱼的同源性相对较低,为86.03%和85.29%。E区域的分析结果表明,鲫IGF-Ⅰ序列为Ea-2亚型。
|
| 关 键 词： | 鲫(Carassius auratus) 胰岛素样生长因子-Ⅰ cDNA RT-PCR 序列分析 |
| 文章编号： | 1000-6907-（2007）04-0024-06 |
| 修稿时间： | 2006-11-20 |
Molecular Cloning and Sequence Analysis of Insulin-like Growth Factor-Ⅰ cDNA from Carassius auratus |
| |
| LIU Jun,CHEN Ai-jing,HUANG Feng,ZHAO Sheng-jun,HU Bing,HOU Yong-qing.Molecular Cloning and Sequence Analysis of Insulin-like Growth Factor-Ⅰ cDNA from Carassius auratus[J].Freshwater Fisheries,2007,37(4):24-29. |
| |
| Authors: | LIU Jun CHEN Ai-jing HUANG Feng ZHAO Sheng-jun HU Bing HOU Yong-qing |
| |
| Institution: | Hubei Key Laboratory of Animal Nutrition and Feed Science, Wuhan Polytechnic University, Wuhan 430023 |
| |
| Abstract: | The insulin-like growth factor Ⅰ(IGF-Ⅰ) cDNA of Carassius auratus was cloned from hepatopancreas by One-Step RT-PCR technique.Nucleotide sequence analysis showed that the IGF-ⅠcDNA of C.auratus was consisted of 486 nucleotides and encoded 161 amino acids including signal peptide,mature peptide(containing B,C,A and D domains)and E domain.Analysis of nucleotide sequence showed that IGF-ⅠcDNA of C.auratus was similar to some other fishes,which shared sequence homology of 99.18% with Carassius auratus Var.,94.44% with Cirrhinus molitorella,94.65% with Megalobrama terminalis,94.44% with Megalobrama amblycephata,86.03% with Cyprinus carpio,and 85.29% with Cterapharyngodon idella.Analysis of E domain indicated that IGF-ⅠcDNA of C.auratus belonged to IGF-ⅠEa-2 subtype. |
| |
| Keywords: | RT-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
