枯草芽孢杆菌基因组DNA对中华绒螯蟹非特异性免疫因子的影响

将枯草芽孢杆菌(Bacillus subtzlis)基因组DNA按5、20和80μg/kg剂量体腔注射中华绒螯蟹(Eriocheir sinensis)(约0.1 mL/只),设注射等体积TE缓冲液为对照组,分别在第1、3、5、7天采样,测定该细菌DNA对中华绒螯蟹的免疫刺激作用。结果显示:注射上述3种剂量的枯草芽孢杆菌基因组DNA均能不同程度地提高中华绒螯蟹的THC、血细胞呼吸爆发活性和血清PO、ACP活性,试验组蟹的上述免疫指标的活性显著高于对照组(P<0.05),并以20μg/kg体重剂量的刺激效果最好;但试验剂量的细菌DNA对血清ALP活性无显著影响(P>0.05)。

Effects of genomic DNA of Bacillus subtilis on non-specific immune factors of Chinese mitten crab (Eriocheir sinensis)

The immtmostimulatory effects of genomic DNA of Bacillus subtilis on Chinese mitten crab (Eriocheir sinensis) were studied when the crabs were injected through coelom by dose of 5,20 and 80 μg/kg body weight (about 0.1 mL/crab) individually,and one group injected with 0.1 mL TE buffer was set as a control.The major immune parameters of E.sinensis were examined at 1,3,5 and 7 days after administration of bacterial DNA or TE buffer.The results showed that three doses of bacterial DNA could enhance THC,respiratory burst of haemocytes,PO and ACP activities in serum of E.sinensis to different extent.The activities of the above immune parameters of crabs in three test groups were significantly higher than those of the control group at most of the sampling time points (P＜0.05).The immunostimulatory effects of bacterial DNA on E.sinensis by injection at 20 μg/kg body weight were the best.And no significant difference on the ALP activities in serum of E.sinensis was detected after the administration of bacterial DNA (P＞0.05).