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厚颌鲂外周血细胞形态及细胞化学特征研究
引用本文:赵娜,杜长雷,金丽,张耀光.厚颌鲂外周血细胞形态及细胞化学特征研究[J].淡水渔业,2011,41(2).
作者姓名:赵娜  杜长雷  金丽  张耀光
作者单位:淡水鱼类资源与生殖发育教育部重点实验室水产科学重庆市市级重点实验室西南大学生命科学学院;
基金项目:公益性行业科研专项(200903048-08)
摘    要:采用血细胞常规染色和细胞化学染色对厚颌鲂(Megalobrama pellegrini)外周血细胞形态及细胞化学特征进行观察。结果显示:厚颌鲂外周血细胞可分为红细胞、血栓细胞、单核细胞、淋巴细胞和三种类型粒细胞。红细胞有幼稚、成熟和衰老三种形态,其中幼稚和衰老红细胞较少见;血栓细胞数量最多,其次是淋巴细胞。粒细胞中Ⅰ型和Ⅱ型较常见,Ⅲ型粒细胞少有。细胞化学染色显示幼红细胞、血栓细胞和淋巴细胞均呈ACP弱阳性;单核细胞呈ACP和ANAE阳性;Ⅰ型粒细胞是嗜中性粒细胞,POX、ACP和ANAE阳性;Ⅱ型粒细胞是PAS-GL,PAS强阳性和ACP阳性;Ⅲ型粒细胞是Ⅰ型粒细胞的晚期形态。厚颌鲂的PAS-GL可能是嗜酸性/嗜碱性粒细胞,而不是嗜碱性粒细胞的前体。

关 键 词:厚颌鲂(Megalobrama  pellegrini)  血细胞  细胞化学

Morphological and cytochemical observations on the peripheral blood cells of Megalobrama pellegrini
ZHAO Na,DU Chang-lei,JIN Li,ZHANG Yao-guang.Morphological and cytochemical observations on the peripheral blood cells of Megalobrama pellegrini[J].Freshwater Fisheries,2011,41(2).
Authors:ZHAO Na  DU Chang-lei  JIN Li  ZHANG Yao-guang
Institution:ZHAO Na,DU Chang-lei,JIN Li,ZHANG Yao-guang(Key Laboratory of Freshwater Fish Reproduction and Development Ministry of Education,The Key Laboratory of Aquatic Science of Chongqing,School of Life Science,Southwest University,Chongqing 400715)
Abstract:The Wright-Giemsa(W-G) and Cytochemical staining methods were used for observations on the morphological features and cytochemical characteristics of blood cells in Megalobrama pellegrini.Erythrocytes,thrombocytes,lymphocytes,monocytes and three type of granulocytes,were identified in the peripheral blood of M.pellegrini.Except mature erythrocytes,immature erythrocytes(IE) and old erythrocytes(OE) were observed,and IE and OE are relatively less;thrombocytes are the most abundant blood cells after erythrocyt...
Keywords:Megalobrama pellegrini  blood cells  cytochemistry  
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