养殖鲈鲤肠道优势菌群组成及来源分析

采用免培养16S rDNA的PCR-DGGE技术,对单一饲料养殖鲈鲤(Percoypris pingi pingi (Tchang))幼鱼(体重为13.61±1.86 g)肠道菌群(包括肠道壁、肠道内容物)、养殖水体、饲料等菌群结构进行了比较分析.结果显示:人工养殖的鲈鲤幼鱼肠道内容物菌群与肠道壁的菌群存在一定的相似性...

Study on composition and generation of predominant intestinal bacteria in cultured Percoypris pingi pingi

The comparative analysis of bacterial community structure in aquaculture water,feed and in the intestinal canal of Percoypris pingi pingi larvae(body weight 13.61±1.86 g),including intestinal contents and intestinal wall,was conducted,based on 16S rDNA PCR-DGGE figure using a culture independent approach.The results showed that the bacterial community displayed a certain similarity between intestinal contents and intestinal wall of the fish under artificial rearing conditions.The bacterial community of intestinal contents was richer than that of intestinal wall.The bacterial communities of intestinal contents in midgut and hindgut were larger than that in foregut,but for intestinal wall there were no significant differences in bacterial communities among the fore-,mid-and hindgut.The main source of gastrointestinal microbiota for the fish was feed.The bacterial communities of intestinal contents and intestinal wall had a greater relevance to the feed,with the values up to 51.6% and 43.0%,respectively.Therefore,it may be viable to add microbial ecological agents in the feed to improve the ability of disease resistance and nutrition intake for the fish.