| 茜素络合物浸泡标记秦岭细鳞鲑发眼卵及仔鱼耳石 |
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| 引用本文： | 靳建波,危起伟,孙庆亮,李罗新,甘芳.茜素络合物浸泡标记秦岭细鳞鲑发眼卵及仔鱼耳石[J].淡水渔业,2011,41(2):10-17. |
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| 作者姓名： | 靳建波 危起伟 孙庆亮 李罗新 甘芳 |
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| 作者单位： | 华中农业大学水产学院;农业部淡水生物多样性保护与利用重点开放实验室中国水产科学研究院长江水产研究所;中国水产科学研究院淡水渔业研究中心; |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金项目(2008JBYWF-16) |
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| 摘 要： | 采用茜素络合物(ALC)在秦岭细鳞鲑(Brachymystax lenok tsinlingensis)发眼卵和仔鱼阶段进行浸泡标记试验,为确定合适浸泡浓度和持续浸泡时间,试验设置了6个的浓度组和4个时间梯度组.结果显示:在荧光显微镜下观察被浸泡的仔鱼和经浸泡处理的发眼卵而出膜后的仔鱼,其耳石都出现橘红色荧光标记.浸泡...
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| 关 键 词： | 秦岭细鳞鲑(Brachymystax lenok tsinlingensis) 茜素络合物 耳石 标记 发眼卵及仔鱼 |
Alizarin marking of otolith at eyed eggs and larvae stages in Brachymystax lenok tsinlingensis |
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| JIN Jian-bo,WEI Qi-wei,SUN Qing-liang,LI Luo-xin,GAN Fang.Alizarin marking of otolith at eyed eggs and larvae stages in Brachymystax lenok tsinlingensis[J].Freshwater Fisheries,2011,41(2):10-17. |
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| Authors: | JIN Jian-bo WEI Qi-wei SUN Qing-liang LI Luo-xin GAN Fang |
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| Institution: | JIN Jian-bo1,2,WEI Qi-wei1,3,SUN Qing-liang1,LI Luo-xin2,GAN Fang2 (1.Fisheries College of Huazhong Agricultural University,Wuhan 430070,2.Key Laboratory of Freshwater Biodiversity Conservation and Utilization,Ministry of Agriculture of China,Yangtze River Fisheries Research Institute,Chinese Academy of Fisheriy Sciences,Wuhan 430223,3.Freshwater Fisheries Research Center,Chinese Academy of Fishery Sciences,Wuxi,Jiangsu 214081) |
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| Abstract: | To study the effects of ALC for marking the eggs and larvae,Brachymystax lenok tsinlingensis eyed eggs and larvae were immersed with twenty-four combinations of six different ALC concentration and four treatment time.The otoliths of larvae were detected by fluorescence microscope.The results showed that the larvae immersed in alizarin complexone solution or which from treated eggs presented distinct scarlet-pink fluorescent marks on otolith under UV light.30 mg/L for 18 h was found to be the most appropriat... |
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| Keywords: | Brachymystax lenok tsinlingensis Alizarin complexone otolith marking eyed eggs and larvae |
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