| 鲫体重和体厚相关的EST-SSRs标记筛选及同源基因分析 |
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| 引用本文： | 郭薇,郑先虎,匡友谊,崔晓亮,曹顶臣,孙效文.鲫体重和体厚相关的EST-SSRs标记筛选及同源基因分析[J].淡水渔业,2013(2):9-15. |
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| 作者姓名： | 郭薇 郑先虎 匡友谊 崔晓亮 曹顶臣 孙效文 |
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| 作者单位： | 哈尔滨师范大学生命科学与技术学院;中国水产科学研究院黑龙江水产研究所农业部淡水水产生物技术与遗传育种实验室;大连海洋大学水产与生命科学学院 |
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| 基金项目： | 公益性行业(农业)科研专项(200903045);国家973计划项目(2010CB126305);农业部948计划项目(2011-G12) |
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| 摘 要： | 利用300个EST-SSRs标记对普通二倍体鲫(Carassius auratus)自交F2代的181个个体进行基因型检测,并对其体重、体厚两种经济性状进行单标记回归分析。Permutation检验(10 000次)结果显示,38个标记与所检测的经济性状相关,其中26个标记达到显著性相关(P<0.05),12个标记达到极显著性相关(P<0.01)。在38个相关标记中,有27个标记与体重相关,25个标记与体厚相关,14个标记与体重、体厚均相关。对同一标记的不同基因型之间进行多重比较,找到了与两种经济性状相关的基因型。将与性状相关的38个标记与斑马鱼(Da-nio rerio)基因组序列进行BLAST同源性比对,找到10条相似性高且功能已知的EST-SSRs序列(E
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| 关 键 词： | 鲫(Carassius auratus) EST-SSRs 体重 体厚 同源比对 |
Screening and homology analysis of EST-SSRs makers related to body weight and body width in Carassius auratus |
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| GUO Wei,ZHENG Xian-hu,KUANG You-yi,CUI Xiao-liang,CAO Ding-chen,SUN Xiao-wen.Screening and homology analysis of EST-SSRs makers related to body weight and body width in Carassius auratus[J].Freshwater Fisheries,2013(2):9-15. |
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| Authors: | GUO Wei ZHENG Xian-hu KUANG You-yi CUI Xiao-liang CAO Ding-chen SUN Xiao-wen |
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| Institution: | 1,2(1.College of Life Science and Technology,Harbin Normal University,Harbin 150025,China; 2.Freshwater Fisheries Biotechnology and Genetic Breeding Key lab of Ministry of Agriculture/ Heilongjiang River Fisheries Research Institute,Chinese Academy of Fisheries Sciencess,Harbin 150070,China; 3.College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,Liaoning,China) |
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| Abstract: | 300 EST-SSRs markers were selected to analyze the genomic DNA of 181 progenies from a normal Carassius auratus F2 family,and two growth traits(body weight and body width) were conducted single marker regression analysis.38 EST-SSRs markers were found to be closely related to growth traits,26 of them were significantly associated with growth traits(P<0.05),the other 12 markers had a highly significant correlation with growth traits(P<0.01);In these 38 markers,27 markers were related with body weight,25 markers were related with body width,and 14 markers were related with both body weight and width.Meanwhile,the genotypes of these correlative loci were determined.Furthermore,by using BLAST online in NCBI,the homology identity of these 38 EST-SSRs markers was determined.Results showed that,10 markers had significant similarity with the sequences in GenBank(E
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| Keywords: | Carassius auratus EST-SSRs body weight body width homology identity |
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