罗非鱼海豚链球菌疫苗及其免疫效果的研究

用筛选到的海豚链球菌(Streptococcus iniae)临床分离菌株CMS005,甲醛灭活制备成疫苗并对其进行了注射、浸泡和口服免疫奥尼罗非鱼的效果研究。结果显示:实验室水族箱试验的注射、浸泡和口服免疫的最佳免疫保护率分别为90.5%、61.9%和14.3%,水泥池小网箱试验的最佳免疫保护率分别为100%、86.1%和66.7%。免疫剂量对浸泡免疫效果的影响大于注射和口服免疫,加强免疫可显著提高浸泡和口服免疫效果,超声波处理可以提高浸泡免疫效果。注射和口服免疫7 d和15 d后均可控制罗非鱼海豚链球菌病的继续发生;未免疫组罗非鱼月累计死亡率为4%~16%,而免疫组罗非鱼月累计死亡率低于0.5%;疫苗产生的免疫保护力可持续2~3个月。

Studies on Streptococcus iniae Vaccine and Its Immune Effect in Tilapia

The immune effect of a vaccine prepared with formalin killed strain CMS005 selected from 3 typical strains of clinic S.iniae in tilapia vaccinated by injection,bath,and oral respectively were studied.The immune experiment showed that the best immune protective rate of three methods were 90.5%,61.9% and 14.3% respectively under laboratory experiment in tank,and the results were 100%,86.1% and 66.7% respectively in small cage in pool.The data analysis revealed the vaecinate dose had greater influence on immune efficacy of bath group than injection and oral group,and the second vaccination can significantly improve the immune efficacy of bath and oral groups.In addition,ultrasonic treatment can improve the protective efficacy of bath group.The field experiment displayed that the S.iniae disease was controlled after vaccinated 7 and 15 days by injection and oral vaccination;the accumulative mortality of non vaccinated group was between 4%～16% in a month,whereas the vaccinated group did not reach 0.5%;the protection duration of the vaccine could last 2～3 months.