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| 呋喃唑酮降解菌株的分离鉴定及降解特性研究 | |
| 引用本文： | 蒋丽娟,张彤晴,杨柳燕,肖琳,潘建林,尹大强.呋喃唑酮降解菌株的分离鉴定及降解特性研究[J].淡水渔业,2007,37(6):36-40. |
| 作者姓名： | 蒋丽娟 张彤晴 杨柳燕 肖琳 潘建林 尹大强 |
| 作者单位： | 1. 污染控制与资源化研究国家重点实验室,南京大学环境学院,南京,210093 2. 江苏省淡水水产研究所,南京,210017 3. 污染控制与资源化研究国家重点实验室,南京大学环境学院,南京,210093;污染控制与资源化研究国家重点实验室,同济大学环境科学与工程学院,上海,200092 |
| 基金项目： | 江苏省科技计划，国家自然科学基金，江苏省科技厅社会发展基金 |
| 摘 要： | 从污染鱼塘底泥中筛选分离出一株能有效降解低浓度呋喃唑酮的细菌F5,经16S rDNA同源性序列分析,鉴定为铜绿假单胞菌(Pseudom onas aeruginosa)。30℃静置避光培养30 d后,该菌株对0.75、1.0和2.5 mg/L呋喃唑酮的降解率分别为58.8%、64.1%和38.2%。温度为20~25℃更有利于该菌株的降解作用,对1.0 mg/L呋喃唑酮降解率均能达85%。碳源和氮源浓度的提高均能促进该菌株的生长和对呋喃唑酮的降解。在0.5 mg/L低磷浓度下,该菌株生长受抑制但降解活性增加,对1.0 mg/L呋喃唑酮降解率达71.8%。 |
| 关 键 词： | 呋喃唑酮降解菌株 铜绿假单胞菌(Pseudomonas aeruginosa) 生物降解 16SrDNA |
| 文章编号： | 1000-6907-（2007）06-0036-05 |
| 修稿时间： | 2007-06-09 |
The Isolation and Identification of Furazolidone-degrading Bacteria F5 and Optimization of Its Degradation Condition |
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| JIANG Li-juan,ZHANG Tong-qin,YANG Liu-yan,XIAO Lin,PAN Jian-lin,YIN Da-qiang.The Isolation and Identification of Furazolidone-degrading Bacteria F5 and Optimization of Its Degradation Condition[J].Freshwater Fisheries,2007,37(6):36-40. | |
| Authors: | JIANG Li-juan ZHANG Tong-qin YANG Liu-yan XIAO Lin PAN Jian-lin YIN Da-qiang |
| Institution: | 1, State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanfing University, Nanfing 210093 ; 2. Freshwater Fisheries Research Institute of Jiangsu Province, Nanfing 210017; 3. State Key Laboratory of Pollution Control and Resources Reuse, College of Environmental Science and Engineering,Tongji University, Shanghai 200092 |
| Abstract: | A Strain of bacterium F5 with high furazolidone degradation was selected from the polluted sediment of a fish pond after tamed and enriched.Through sequence analysis of the 16S rDNA,F5 was identified as Pseudomonas aeruginosa.When F5 was incubated in enrichment liquid medium which contained 0.75,1.0,2.5 mg/L furazolidone respectively,the degradation rates were 58.8%,64.1% and 38.2% respectively after 30 d at 30 ℃.The optimum temperature for biodegradation was 20～25 ℃ and the degradation rate for 1.0 mg/L furazolidone was about 85 %.Glucose and nitrogen can improve furazolidone degradation.Phosphorous can inhibit the growth of F5,but the activity of strain was improved,and the degradation rate was 71.8%. |
| Keywords: | 16S rDNA |
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