甲基睾酮免疫胶体金试纸条的研制及在水产品中的应用

通过研究胶体金免疫层析技术,建立了一种快速检测水产品中甲基睾酮含量的方法。将待测样品中的甲基睾酮和试纸条检测线上包被的人工抗原(MT-BSA)竞争性结合金标结合垫上包被的胶体金-甲基睾酮单抗聚合物,根据检测线呈现的红色线条深浅来判定样品的阴阳性。结果显示:试纸条上MT-BSA抗原工作浓度为1.0mg/m L,羊抗鼠二抗工作浓度为1.5 mg/m L,试纸条的最低检测限达到10μg/kg,与甲基睾酮类似物的交叉反应率低;使用甲醇提取,4倍PBST稀释,整个检测所需时间仅10~15 min,适用于大规模样品筛选。

Development of a coloidal gold immunochromatographic strip for methyltestosterone residue in aquatic products

A rapid coloidal gold immunochromatographic assay for methyltestosterone ( MT) residue in aquatic products was developed.The coloidal gold-MT-McAb conjugate coated on the colloidal gold conjugate pad would combine with the MT in the samples and the MT-BSA on the detective line competitively, thus indicated the result of test.The results of this article showed that working concentration of MT-BSA was 1.0 mg/mL and goat anti-mouse IgG was 1.5 mg/mL, the detec-tion limit of the strip could be 10 μg/kg, and the cross-reactivity was low, MT was extracted by methanol, and then the extract was diluted by 4 times PBST solution, the result could be obtained in 10~15 min.Therefore the strip was suitable for the sample screening and qualitative determination.