| 黄河鲶不同组织中RNA提取纯化方法研究 |
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| 引用本文： | 吴旭东,侯玉霞,张文吉.黄河鲶不同组织中RNA提取纯化方法研究[J].淡水渔业,2006,36(2):24-26. |
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| 作者姓名： | 吴旭东 侯玉霞 张文吉 |
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| 作者单位： | 中国农业大学理学院,北京,100094 |
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| 基金项目： | 宁夏科技兴农计划(8613计划) |
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| 摘 要： | 从组织提取RNA的完整性对于分子生物学研究是至关重要的。RNA提取方法有多种。采取异硫氰酸胍法从黄河鲶肌肉、血液、全鱼、肾脏、肝脏、卵巢中提取出完整RNA,结果表明,通过该方法所提取RNA质量高,效果好,可以用于进一步的分子生物学研究。
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| 关 键 词： | 黄河鲶 RNA提取 异硫氰酸胍法 |
| 文章编号： | 1000-6907-(2006)02-0024-03 |
| 收稿时间： | 2005-12-27 |
| 修稿时间： | 2005年12月27 |
The Method of Extracting and Purifying RNA from Different Tissues of the Yellow River Catfish |
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| WU Xu-dong,HOU Yu-xia,ZHANG Wen-ji.The Method of Extracting and Purifying RNA from Different Tissues of the Yellow River Catfish[J].Freshwater Fisheries,2006,36(2):24-26. |
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| Authors: | WU Xu-dong HOU Yu-xia ZHANG Wen-ji |
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| Institution: | College of Science, China Agricultural University, Beijing 100094 |
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| Abstract: | The integrality of RNA extracted and purified from tissues is essential for molecular biology.Some useful extracting and purifying methods had been discovered.Guanidine Thiocyanate is ideal for extracting and purifying RNA of the Yellow River catfish.Through this method,RNA of high quality had been obtained from muscle,blood,kidney,liver,ovary,which can be used for the further molecular biology research. |
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| Keywords: | the Yellow River catfish RNA extraction Guanidine Thiocyanate |
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