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星洲红鱼形态、染色体组型及细胞核DNA含量的分析
引用本文:黄永春,李文静,林祥日,蔡明夷,许洪玉,李雅玲.星洲红鱼形态、染色体组型及细胞核DNA含量的分析[J].淡水渔业,2011,41(5):3-8.
作者姓名:黄永春  李文静  林祥日  蔡明夷  许洪玉  李雅玲
作者单位:1. 集美大学水产学院,福建厦门,361021
2. 厦门海洋职业技术学院,福建厦门,361012
3. 集美区农业局水产技术推广站,福建厦门,361021
基金项目:李尚大集美大学学科建设基金,福建省发改委"农业创新工程"项目,集美区科技局项目
摘    要:对星洲红鱼(Red Tilapia)形态特征、染色体组型及细胞核DNA含量的研究结果表明:星洲红鱼体型侧扁,体色鲜红;星洲红鱼鳍式:背鳍XⅥ~XⅦ,12 ~ 14;胸鳍13 ~ 14;腹鳍1,5;臀鳍Ⅲ,9~11星洲红鱼体被硬圆鳞,侧线鳞数28~30,上侧线鳞数21~23,下侧线鳞数14 ~16;采用PHA、秋水仙碱腹...

关 键 词:星洲红鱼(RedTilapia)  形态特征  染色体  核型  DNA含量

Morphology, chromosome karyotype and nuclear DNA contents of Red Tilapia
HUANG Yong-chun,LI Wen-jing,LIN Xiang-ri,CAI Ming-yi,XU Hong-yu,LI Ya-ling.Morphology, chromosome karyotype and nuclear DNA contents of Red Tilapia[J].Freshwater Fisheries,2011,41(5):3-8.
Authors:HUANG Yong-chun  LI Wen-jing  LIN Xiang-ri  CAI Ming-yi  XU Hong-yu  LI Ya-ling
Abstract:The morphological characteristics,chromosome karyotype and nuclear DNA contents of Red Tilapia were studied in this experiment,the results showed that Red Tilapia was short and high with red or orange stripe.Its fin-type:Dorsal finⅩⅥ~ⅩⅦ,12~14;Pectoral fin13~14;Abdomen finⅠ,5;Anal finⅢ,9~11.Lateral line scales of Red Tilapia:28~30,whose above lateral line scales 21~23 and follow lateral line scales 14~16.The metaphase chromosome of Red Tilapia was prepared from kidney by PHA and colchicine injection and air drying.The karyotype was analyzed.The results showed that there were 44 telocentric chromosomes in Red Tilapia with a large and long chromosome pair,and no metacentric chromosome pairs and heteromorphic chromosomes were found.Its karyotype was 2n=6sm+4st+34t(NF=50),which relative length of chromosome was 1.58%~4.98%.Using DNA of chicken erythrocyte(2.50 pg/2c) as a standard,diploid nucleus DNA contents of Red Tilapia were measured from erythrocyte by flow cytometer(EPICS-XL).DNA content was(2.5354 9±0.1152)pg/2c.
Keywords:Red Tilapia  morphological characteristics  chromosome  karyotype  DNA content
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