| 大孔吸附树脂纯化生姜提取物中6-姜酚工艺优化 |
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| 引用本文： | 刘伟,周春丽,赵婧,陈冬,李全宏.大孔吸附树脂纯化生姜提取物中6-姜酚工艺优化[J].农业机械学报,2014,45(6):237-241,328. |
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| 作者姓名： | 刘伟 周春丽 赵婧 陈冬 李全宏 |
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| 作者单位： | 中国农业大学;中国农业大学;江西科技师范大学;中国农业大学;中国农业大学;中国农业大学 |
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| 基金项目： | 国家重点基础研究发展计划（973计划）资助项目（2012CB720805）和“十二五”国家科技支撑计划资助项目（2011BAD23B01） |
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| 摘 要： | 比较了6种大孔吸附树脂对6-姜酚的吸附和解吸性能,在静态吸附研究基础上筛选出效果较好的树脂进行动态试验,并通过HPLC-UV和GC-MS对纯化前、后的成分进行了定性和定量分析。结果表明:D101型大孔树脂更适合纯化姜油树脂中的6-姜酚,其纯化的最佳工艺条件为:上样液质量浓度2 mg/mL,流速2 mL/min,3 BV去离子水洗脱,然后用体积分数80%乙醇以2 mL/min的流速洗脱4 BV,可以使6-姜酚的纯度从1.54%提高到71.32%。
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| 关 键 词： | -姜酚 大孔树脂 纯化 |
| 收稿时间： | 2013/12/6 0:00:00 |
Purification of 6-gingerol from Oleoresin with Macroporous Resin |
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| Liu Wei,Zhou Chunli,Zhao Jing,Chen Dong and Li Quanhong.Purification of 6-gingerol from Oleoresin with Macroporous Resin[J].Transactions of the Chinese Society of Agricultural Machinery,2014,45(6):237-241,328. |
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| Authors: | Liu Wei Zhou Chunli Zhao Jing Chen Dong and Li Quanhong |
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| Institution: | China Agricultural University;China Agricultural University;Jiangxi Science and Technology Normal University;China Agricultural University;China Agricultural University;China Agricultural University |
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| Abstract: | In order to separate and purify 6-gingerol from oleoresin by macroporous resin, 6 kinds of macroporous resins were chosen to carry out static and dynamic adsorption-desorption experiments. Qualitative and quantitative analysis of purification results were analyzed by HPLC-UV and GC-MS. The results showed that D101 was the optimal macroporous resin for separating and purifying 6-gingerol, the best technology parameters were as follows: sample concentration of 2 mg/mL, distilled water of 3 BV, 80% ethanol for elution velocity of 2 mL/min, elution volume of 4BV. Under these conditions, the purity of 6-gignerol enhanced from 1.54% to 71.32%. |
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